| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
DNA-conjugated stimuli-responsive materials coordinate the molecular recognition properties of DNA and actuator properties of thermoresponsive polymer, poly(N-isopropylacrylamide) (pNIPAM). In some specific conditions, DNA-pNIPAM changes its dispersion state in response to whether conjugated DNA is single-stranded (ss) or double-stranded (ds). Under the condition where entropic repulsion controls the aggregation behavior of the conjugate, the aggregation was controlled by whether the dangling end of dsDNA had a specific structure (e.g. G-quadruplex) or not. Then, this property was combined with the molecular recognition of a DNA aptamer. The target protein, thrombin, bound to DNA-pNIPAM, and this binding changed the aggregation behavior of the conjugate.
|
