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Construction of heat-resistant ethanol-fermenting fungus by ion beam mutation and its application to consolidated bioprocessing

Research Project

Project/Area Number 15K06577
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Toyama

Principal Investigator

HOSHINO Kazuhiro  富山大学, 大学院理工学研究部(工学), 准教授 (20222276)

Research Collaborator HATASHITA Masanori  (公財)若狭湾エネルギー研究センター, 主任研究員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords耐熱菌 / 糸状菌 / エタノール発酵 / イオンビーム / 変異株 / 放射線変異 / バイオマス / 耐熱性菌 / イオンビーム照射 / 変異誘導 / イオンビーム変異 / セルラーゼ
Outline of Final Research Achievements

In order to produce directly ethanol from unused lignocellulose resources, a heat-resistant ethanol fermented filamentous fungus mutant strain which increased fermentable temperature of ethanol fermented filamentous fungus and improved cellulase secretion ability by carbon ion beam mutagenesis with a dose of 2,500 Gy, was constructed. By CBP process utilizing this strain, we successfully produced ethanol directly from α-cellulose in an environment at 45℃.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (22 results)

All 2018 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (21 results) (of which Int'l Joint Research: 11 results,  Invited: 1 results)

  • [Journal Article] Bioethanol production from rice straw by simultaneous saccharification and fermentation with statistical optimized cellulase cocktail and fermenting fungus2018

    • Author(s)
      Maki Takano, Kazuhiro Hoshino
    • Journal Title

      Bioresources and Bioprocessing

      Volume: 5 Issue: 1 Pages: 16-16

    • DOI

      10.1186/s40643-018-0203-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] イオンビーム育種による新規発酵プロセスのための高機能糸状菌の開発2017

    • Author(s)
      星野 一宏, 高野 真希, 畑下 昌範
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] イオンビーム変異誘導法によるCullulase分泌耐熱性Ethanol生産糸状菌の構築2017

    • Author(s)
      山下 聖樹, 高野 真希, 星野 一宏
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] イオンビーム変異誘導法による耐熱性乳酸生産糸状菌のXylose代謝向上2017

    • Author(s)
      高野 真希, 畑下 昌範, 星野 一宏
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Elucidation of Dimorphic Mechanism by Intracellular cAMP on Mucor circinellides2017

    • Author(s)
      Ryo Iwakura, Maki Takano and Kazuhiro Hoshino
    • Organizer
      The 13th Asian Congress on Biotechnology 2017 (ACB 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Direct Production of L-Malic Acid from Lignocellulose with S. commume Mutant Constructed by Ion-beam Irradiation2017

    • Author(s)
      Kazuhiro Hoshino, Maki Takano and Masanori Hatashita
    • Organizer
      The 13th Asian Congress on Biotechnology 2017 (ACB 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High Temperature Ethanol Production from Rice Straw by Cellulase Secreting Fungi Stimulated by Ion-beam Mutation2017

    • Author(s)
      Maki Takano, Satoki Yamashita, Masanori Hatashita and Kazuhiro Hoshino
    • Organizer
      The 13th Asian Congress on Biotechnology 2017 (ACB 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 耐熱性乳酸糸状菌のイオンビーム変異誘導によるXylose発酵性向上における機能解析2017

    • Author(s)
      髙野真希、山下聖樹、畑下昌範、星野一宏
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] イオンビーム照射による直接Ethanol生産のためのCellulase分泌糸状菌の構築2017

    • Author(s)
      山下聖樹、髙野真希、畑下昌範、星野一宏
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Production and Characteristics of High-active Cellulase Derived from Schizopullum commune for Efficient Hydrolysis of Lignocellulase2017

    • Author(s)
      Kazuhiro Hoshino, Maki Takano and Masanori Hatashita
    • Organizer
      The 4th International Cellulose Conference (ICC2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Constraction of a High-performing Fungus by Ion-beam Irradiation for Ethanol Production from Cellulosic materials2017

    • Author(s)
      Satoki Yamashita, Maki Takano, Masanori Hatashita, Kazuhiro Hoshino
    • Organizer
      The 4th International Cellulose Conference (ICC2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Direct prodcution of L-malic acid and ethanol from lignocellulose by consolidated bioprecessing process with Schizophyllum commune mutant2016

    • Author(s)
      K.Hoshino, M.Takano. M.Hatashita,
    • Organizer
      17th International Biotechnology Symposium and Exhibition (IBS 2016),
    • Place of Presentation
      メルボルン国際会議場
    • Year and Date
      2016-10-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Construcation of a xylose-fanetable mutant from thermotolerant lactic acid producing fungi by ion-beam irradiation2016

    • Author(s)
      S.Yamashita, M.Takano, M.Hatashita, K.Hoshino
    • Organizer
      17th International Biotechnology Symposium and Exhibition (IBS 2016),
    • Place of Presentation
      メルボルン国際会議場
    • Year and Date
      2016-10-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bioethanol pruduction from paler sludge by novel thermotolerant ehtaol producing-fungus2016

    • Author(s)
      M.Takano, M.Hatashita, K.Hoshino
    • Organizer
      17th International Biotechnology Symposium and Exhibition (IBS 2016),
    • Place of Presentation
      メルボルン国際会議場
    • Year and Date
      2016-10-24
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] イオンビーム変異法による耐熱性乳酸生産糸状菌のXylose発酵変異株の構築2016

    • Author(s)
      山下 聖樹, 高野 真希, 畑下 昌範, 星野 一宏
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 二形性調節因子による酵母化Mucor circinelloidesの構築および好気的Ethanol生産2016

    • Author(s)
      岩倉 遼, 高野 真希, 星野 一宏
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 耐熱性Rhizopus属糸状菌を用いた高温同時糖化発酵によるセルロース系バイオマスからの乳酸生産2016

    • Author(s)
      高野真希、山下 聖樹、星野 一宏
    • Organizer
      日本農芸化学会2016大会
    • Place of Presentation
      札幌コンベンションセンター、札幌
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] イオンビームによる耐熱性乳酸生産糸状菌のXylose発酵変異株の構築およびリグノセルロースからの同時糖化発酵への応用2016

    • Author(s)
      山下聖樹、高野真希、畑下昌範、星野 一宏
    • Organizer
      日本農芸化学会2016大会
    • Place of Presentation
      札幌コンベンションセンター、札幌
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Bioethanol production from CaCO3-removed paper sludge by thermotolerant fermenting-fungus2015

    • Author(s)
      Maki Takano, Kazuhiro Hoshino
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lactic Acid Prodcution from Paper Sludge by SSF with Thermotolerant Rhizopus sp.2015

    • Author(s)
      Maki Takano, Kazuhiro Hoshino
    • Organizer
      Asian Congress on Biotechology 2015 (ACB2015)
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Year and Date
      2015-11-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Construction of Thermotolerant Ethanol Producing Fungi by Ion-Beam Mutation and Its Application to Simultaneous Saccharification and Fermenatation of Lignocellulose2015

    • Author(s)
      Kiichi Tsunoda, Maki Takano, Kazuhiro Hoshino
    • Organizer
      Asian Congress on Biotechology 2015 (ACB2015)
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Year and Date
      2015-11-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] イオンビームへ員方による高熱耐性エタノール発酵糸状菌の構築2015

    • Author(s)
      星野一宏、高野真希、畑下昌範
    • Organizer
      第67回 日本生物工学会大会
    • Place of Presentation
      城山観光ホテル、鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2021-01-27  

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