| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The central carbon metabolism in budding yeast (Saccharomyces cerevisiae) was engineered to improve the production of butanols from biomass. For the purpose, a precise characterization of yeast metabolism was performed by developing a method to determine concentration of intracellular metabolites as well as to estimate levels of Gibb’s free energy change of metabolic reactionss. The results of the precise analysis suggested that an activation of excess NADH consumption was essential with considering intracellular compartmentalization of yeast cells. Based on the findings, the isobutanol production by yeast was improved by an overexpression of mitochondrial pyruvate carrier proteins.
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