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Metabolic Engineering of yeast strains producing higher alcohols based on the precise metabolic characterization

Research Project

Project/Area Number 15K06579
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka University

Principal Investigator

Matsuda Fumio  大阪大学, 情報科学研究科, 教授 (50462734)

Co-Investigator(Renkei-kenkyūsha) ISHII Jun  神戸大学, 大学院・科学技術イノベーション研究科, 教授 (40512546)
Research Collaborator MORITA Keisuke  
NISHINO Shunsuke  
NOMURA Yuta  
KINOSHITA Shohei  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords酵母 / 高級アルコール / 代謝工学 / メタボローム分析 / プロテオーム解析 / 出芽酵母 / 代謝解析 / メタボローム解析
Outline of Final Research Achievements

The central carbon metabolism in budding yeast (Saccharomyces cerevisiae) was engineered to improve the production of butanols from biomass. For the purpose, a precise characterization of yeast metabolism was performed by developing a method to determine concentration of intracellular metabolites as well as to estimate levels of Gibb’s free energy change of metabolic reactionss. The results of the precise analysis suggested that an activation of excess NADH consumption was essential with considering intracellular compartmentalization of yeast cells. Based on the findings, the isobutanol production by yeast was improved by an overexpression of mitochondrial pyruvate carrier proteins.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (10 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Expression of Saccharomyces cerevisiae cDNAs to enhance the growth of non-ethanol-producing S. cerevisiae strains lacking pyruvate decarboxylases2018

    • Author(s)
      Yuki Narazaki, Yuta Nomura, Keisuke Morita, Hiroshi Shimizu, and Fumio Matsuda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 125 Issue: 3 Pages: 317-321

    • DOI

      10.1016/j.jbiosc.2018.03.008

    • NAID

      40021667154

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted proteome analysis of single-gene deletion strains of Saccharomyces cerevisiae lacking enzymes in the central carbon metabolism2017

    • Author(s)
      Matsuda Fumio、Kinoshita Syohei、Nishino Shunsuke、Tomita Atsumi、Shimizu Hiroshi
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 2 Pages: e0172742-e0172742

    • DOI

      10.1371/journal.pone.0172742

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heterologous expression of bacterial phosphoenol pyruvate carboxylase and Entner?Doudoroff pathway in Saccharomyces cerevisiae for improvement of isobutanol production2017

    • Author(s)
      Morita Keisuke、Nomura Yuta、Ishii Jun、Matsuda Fumio、Kondo Akihiko、Shimizu Hiroshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 124 Issue: 3 Pages: 263-270

    • DOI

      10.1016/j.jbiosc.2017.04.005

    • NAID

      40021323422

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose2016

    • Author(s)
      Li Shen, Yuya Nishimura, Fumio Matsuda, Jun Ishii, and Akihiko Kondo
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 122 Issue: 1 Pages: 34-39

    • DOI

      10.1016/j.jbiosc.2015.12.022

    • NAID

      40020885810

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regular expressions of MS/MS spectra for partial annotation of metabolite features2016

    • Author(s)
      Fumio Matsuda
    • Journal Title

      Metabolomics

      Volume: 12 Issue: 7 Pages: 1-14

    • DOI

      10.1007/s11306-016-1052-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Technical Challenges in Mass Spectrometry-Based Metabolomics2016

    • Author(s)
      Fumio Matsuda
    • Journal Title

      Mass Spectrometry

      Volume: 5 Issue: 2 Pages: S0052-S0052

    • DOI

      10.5702/massspectrometry.S0052

    • NAID

      130005285734

    • ISSN
      2186-5116, 2187-137X
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 13C-metabolic flux analysis in S-adenosyl-L-methionine production by Saccharomyces cerevisiae.2015

    • Author(s)
      Kenshi Hayakawa, Shuichi Kajihata, Fumio Matsuda, Hiroshi Shimizu
    • Journal Title

      Journal of Bioscience and Bioengneering

      Volume: 120 Issue: 5 Pages: 532-8

    • DOI

      10.1016/j.jbiosc.2015.03.010

    • NAID

      210000005141

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae2015

    • Author(s)
      Kengo Ida, Jun Ishii, Fumio Matsuda, Takashi Kondo, Akihiko Kondo
    • Journal Title

      Microbial Cell Factories

      Volume: 14 Issue: 1 Pages: 62-62

    • DOI

      10.1186/s12934-015-0240-6

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ピルビン酸代謝フローの転換による出芽酵母2,3-ブタンジオール生産性の向上2017

    • Author(s)
      森田 啓介、松田 史生、岡本 浩二、石井 純、近藤 昭彦、清水 浩
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マイトファジー抑制による出芽酵母の細胞質物質生産能力向上2017

    • Author(s)
      森田 啓介、松田 史生、岡本 浩二、石井 純、近藤 昭彦、清水 浩
    • Organizer
      日本農芸化学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マイトファジー抑制による出芽酵母の細胞質物質生産能力向上2017

    • Author(s)
      森田 啓介、松田 史生、岡本 浩二、石井 純、近藤 昭彦、清水 浩
    • Organizer
      日本農芸化学会 2017 大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2016 Research-status Report
  • [Presentation] 定量プロテオミクスを用いた出芽酵母中心代謝安定化機構の解析2016

    • Author(s)
      松田史生、富田淳美、清水浩
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] イソブタノール生産酵母の構築:オルガネラ局在の検討2016

    • Author(s)
      森田 啓介、松田 史生、伊田賢吾、石井 純、近藤 昭彦、清水 浩
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Enzyme co-expression analysis revealed a metabolic safety device on the central metabolism of Saccharomyces cerevisiae2016

    • Author(s)
      Fumio Matsuda, Atsumi Tomita, and Hiroshi Shimizu
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      淡路夢舞台
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Engineering of mitochondrial isobutanol production in Saccharomyces cerevisiae2016

    • Author(s)
      Keisuke Morita, Fumio Matsuda, Koji Okamoto, Kengo Ida, Jun Ishii, Akihiko Kondo, Hiroshi Shimizu
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      淡路夢舞台
    • Year and Date
      2016-09-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Heterologous gene expression in Saccharomyces cerevisiae for higher isobutanol production2016

    • Author(s)
      Keisuke MORITA, Fumio MATSUDA, Jun ISHII, Akihiko KONDO, Hiroshi SHIMIZU
    • Organizer
      Metabolic Engineering XI
    • Place of Presentation
      淡路夢舞台
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 2,3-ブタンジオール高生産出芽酵母株の構築2016

    • Author(s)
      松田史生、伊田賢吾、木下翔平、森田啓介、石井寛子、石井純、近藤昭彦、清水浩
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 細胞質バリン生合成経路の異種発現による出芽酵母イソブタノール生産性の向上2016

    • Author(s)
      森田啓介、松田史生、石井純、近藤昭彦、清水 浩
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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