Technology with glassin, a protein accelerating silica polycondensation, for development of environmentally benign routes to material synthesis

• Project/Area Number: 15K06581
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tottori University
• Principal Investigator: Shimizu Katsuhiko 鳥取大学, 地域価値創造研究教育機構, 准教授 (90326877)
• Co-Investigator(Kenkyū-buntansha): 有馬 二朗 鳥取大学, 農学部, 准教授 (80393411)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオミネラリゼーション / 海綿動物 / シリカ / 環境調和型 / 生物模倣 / ヒスチジン / 酵素固定化 / グラシン / アミノペプチダーゼ / 人工遺伝子 / 緑色蛍光タンパク質 / グルタチオンS-トランスフェラーゼ / バイオシリカ / カイロウドウケツ

Outline of Final Research Achievements

In this study, we identified the active region in silica formation accelerating protein glassin isolated from the silica skeleton of the hexactinellid sponge Euplectella. We also established a technique to immobilize useful proteins on silica with maintaining their activity by preparing recombinant proteins using glassin as a tag. This achievement meets the original objective, which open a new route for the industrial use of glassin. In addition, we uncovered a new function of glassin which binds strongly to metal ions.

Research Products

• [Int'l Joint Research] CSIC(スペイン)
• [Journal Article] Exploration of genes associated with sponge silicon biominerals in the whole genome sequence of the hexactinellid Euplectella curvistellata (2018)
  • Author(s): Shimizu, H. Kobayashi, T. Amano, M. Nishi, M. Tsukahara, T. Bito, J. Arima
  • Journal Title: Proceedings of 14th International Symposium on Biomineralization Volume: -
  • Peer Reviewed
• [Journal Article] Methionine residues lining the substrate pathway in prolyl oligopeptidase from Pleurotus eryngii play an important role in substrate recognition. (2018)
  • Author(s): S. Tokai, T. Bito, K. Shimizu, J. Arima
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: - Issue: 7 Pages: 1107-1115
  • DOI: 10.1080/09168451.2018.1459177
  • Peer Reviewed
• [Journal Article] Active site pocket of Streptomyces D-stereospecific amidohydrolase has functional roles in aminolysis activity. (2018)
  • Author(s): YY. Ahmed Elyas, K. Miyatani, T. Bito, M. Uraji, T. Hatanaka, K. Shimizu, J. Arima
  • Journal Title: Journal of Bioscience and Bioengineering Volume: - Issue: 3 Pages: 293-300
  • DOI: 10.1016/j.jbiosc.2018.03.004
  • NAID: 210000005757
  • Peer Reviewed
• [Journal Article] Effect of oxidation of the non-catalytic b-propeller domain on the substrate specificity of prolyl oligopeptidase from Pleurotus eryngii (2017)
  • Author(s): Shota Tokai, Tomohiro Bito, Katsuhiko Shimizu, Jiro Arima
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 487 Issue: 2 Pages: 356-361
  • DOI: 10.1016/j.bbrc.2017.04.064
  • Peer Reviewed
• [Journal Article] Plant opal-mimetic bunching silica nanoparticles mediated by long-chain polyethyleneimine (2016)
  • Author(s): Hoshino T, Sato K, Oaki Y, Sugawara-Narutaki A, Shimizu K, Ozaki N, Imai H
  • Journal Title: RSC Advances Volume: 6 Issue: 2 Pages: 1301-1306
  • DOI: 10.1039/c5ra25742e
  • Peer Reviewed
• [Journal Article] Crystal structure of D-stereospecific amidohydrolase from Streptomyces sp. 82F2: insight into the structural factors for substrate specificity. (2016)
  • Author(s): Arima J, Shimone K, Miyatani K, Tsunehara Y, Isoda Y, Hino T, Nagano S
  • Journal Title: FEBS Journal Volume: 283 Issue: 2 Pages: 337-349
  • DOI: 10.1111/febs.13579
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Glassin, a histidine-rich protein from the siliceous skeletal system of the marine sponge Euplectella, directs silica polycondensation (2015)
  • Author(s): Shimizu K, Amano T, Bari MR, Weaver JC, Arima J, Mori N
  • Journal Title: Proc Natl Acad Sci U S A Volume: 112 Pages: 11449-11454
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Proteomic analysis of homocholine catabolic pathway in Pseudomonas sp. strain A9 (2015)
  • Author(s): Mohamed Ahmed IA, Eltayeb ME, Mori N, Arima J, Tanaka H, Taniguchi T, Yamanaka N
  • Journal Title: Process Biochemistry Volume: 50 Issue: 11 Pages: 1735-1747
  • DOI: 10.1016/j.procbio.2015.07.001
  • Peer Reviewed / Int'l Joint Research
• [Presentation] シリカ粒子形成促進タンパク質“グラシン”の構造と性質 (2018)
  • Author(s): 西美智佳，小林大起，美藤友博，有馬二朗，清水克彦
  • Organizer: 2018年度日本農芸化学会中四国支部例会
• [Presentation] Development of method for protein Immobilization on silica for use in food processing industry. (2017)
  • Author(s): H. Kobayashi, M. Nishi, T. Bito, K. Shimizu, J. Arima
  • Organizer: he International Joint Symposium between Japan and Korea (AFELiSA) 2017
• [Presentation] 着生とバイオミネラリゼーション (2017)
  • Author(s): 清水克彦
  • Organizer: 日本付着生物学会シンポジウム
  • Invited
• [Presentation] Exploration of genes associated with sponge silicon biominerals in the whole genome sequence of the hexactinellid Euplectella curvistellata (2017)
  • Author(s): Shimizu, H. Kobayashi, T. Amano, M. Nishi, M. Tsukahara, T. Bito, J. Arima
  • Organizer: 14th International Symposium on Biomineralization
  • Int'l Joint Research
• [Presentation] シリカ粒子形成促進タンパク質“グラシン”の機能によるGST固定シリカ粒子の構築 (2017)
  • Author(s): 小林大起，西美智佳，天野太郎，美藤友博，清水克彦，有馬二朗
  • Organizer: 2017年度日本生物工学会全国大会
• [Presentation] Silicatein and glassin: molecular properties of the proteins occluded in sponge biosilica and their biotechnological implications. (2017)
  • Author(s): K. Shimizu, H. Kobayashi, T. Amano, M. Nishi, T. Bito, J. Arima
  • Organizer: Silicon, Silica & their Isotopes: Biology, Biogeochemistry & Biotechnology 2017
  • Int'l Joint Research
• [Presentation] Immobilization of green fluorescent protein to silica using the function of “glassin”, a silica particle formation promoting protein from hexactinellid sponges. (2017)
  • Author(s): H. Kobayashi, T. Amano, T. Bito, J. Arima, K. Shimizu
  • Organizer: Silicon, Silica & their Isotopes: Biology, Biogeochemistry & Biotechnology 2017
  • Int'l Joint Research
• [Presentation] 海綿動物のシリカバイオミネラル形成タンパク質に関する研究 (2017)
  • Author(s): 清水克彦
  • Organizer: 平成29年度マリンバイオテクノロジー学会大会
  • Invited
• [Presentation] 海綿動物のシリカバイオミネラル形成タンパク質に関する研究 (2017)
  • Author(s): 清水克彦
  • Organizer: 第31回 海洋生物活性談話会
• [Presentation] 海綿動物に学ぶガラス細工 (2017)
  • Author(s): 清水克彦
  • Organizer: 日本農芸化学会中四国支部第25回若手研究者シンポジウム
  • Invited
• [Presentation] Construction of immobilized protein by using the function of silica particle formation promoting protein "glassin" (2016)
  • Author(s): Hiroki Kobayashi, Taro Amano, Tomohiro Bito, Katsuhiko Shimizu, Jiro Arima
  • Organizer: The International Joint Symposium between Japan and Korea (AFELiSA) 2016
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 2016-11-09
  • Int'l Joint Research
• [Presentation] シリカ粒子形成促進タンパク質“グラシン”の機能を利用したGFPのシリカへの固定化 (2016)
  • Author(s): 小林 大起，天野 太郎，有馬 二朗，清水 克彦
  • Organizer: 2016年度日本生物工学会全国大会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-28
• [Presentation] 六放海綿類カイロウドウケツのシリカバイオミネラリゼーションに関わる タンパク質グラシン (2016)
  • Author(s): 清水克彦
  • Organizer: 第11回化学生態学研究会
  • Place of Presentation: 函館
  • Year and Date: 2016-07-01
  • Invited
• [Presentation] 海綿動物のシリコンバイオミネラルに学ぶシリコンマリンバイオテクノロジー (2016)
  • Author(s): 清水克彦
  • Organizer: 第18回マリンバイオテクノロジー学会
  • Place of Presentation: 函館
  • Year and Date: 2016-05-28
  • Invited
• [Presentation] バイオシリカとその形成機構 (2016)
  • Author(s): 清水克彦
  • Organizer: 第20回規則性多孔体セミナー
  • Place of Presentation: 鳥取
  • Invited
• [Presentation] Glassin, a silica polycondensation directing protein from the siliceous skeleton of the hexactinellid sponge Euplectella (2015)
  • Author(s): Shimizu K, Amano T, Arima J
  • Organizer: PacifiChem 2015
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] Characterization of recombinant glassin, a protein extracted from biosilica skeleton of the glass sponge Euplectella (2015)
  • Author(s): Amano T, Arima J, Shimizu K
  • Organizer: PacifiChem 2015
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] Construction of non-natural dipeptide library by peptide bond formation reaction catalyzed by D-stereospecific amidohydrolase. (2015)
  • Author(s): Tsunehara Y, Tamura A, Isoda Y, Arima J
  • Organizer: PacifiChem 2015
  • Place of Presentation: Honolulu, HI, USA
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] カイロウドウケツシリカ骨格に含まれるタンパク質の検索 (2015)
  • Author(s): 清水克彦
  • Organizer: 第86回日本動物学会大会
  • Place of Presentation: 新潟市
  • Year and Date: 2015-09-15
• [Book] Methods in Enzymology Volume 605. Marine Enzymes and Specialized Metabolism - Part B (2018)
  • Author(s): Shimizu K, Morse DE
  • Total Pages: 412
  • Publisher: Elsevier
  • ISBN: 9780128150450