Technology with glassin, a protein accelerating silica polycondensation, for development of environmentally benign routes to material synthesis
| Project/Area Number |
15K06581
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tottori University |
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Principal Investigator |
Shimizu Katsuhiko 鳥取大学, 地域価値創造研究教育機構, 准教授 (90326877)
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| Co-Investigator(Kenkyū-buntansha) |
有馬 二朗 鳥取大学, 農学部, 准教授 (80393411)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バイオミネラリゼーション / 海綿動物 / シリカ / 環境調和型 / 生物模倣 / ヒスチジン / 酵素固定化 / グラシン / アミノペプチダーゼ / 人工遺伝子 / 緑色蛍光タンパク質 / グルタチオンS-トランスフェラーゼ / バイオシリカ / カイロウドウケツ |
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| Outline of Final Research Achievements |
In this study, we identified the active region in silica formation accelerating protein glassin isolated from the silica skeleton of the hexactinellid sponge Euplectella. We also established a technique to immobilize useful proteins on silica with maintaining their activity by preparing recombinant proteins using glassin as a tag. This achievement meets the original objective, which open a new route for the industrial use of glassin. In addition, we uncovered a new function of glassin which binds strongly to metal ions.
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Report
(4 results)
Research Products
(29 results)
