| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Shinella sp. NN-6 produced L-rhamnose isomerase and ketose 3-epimerase when it cultivated using L-rhamnose as a carbon source. Rare sugars, D-psicose and D-allose were produced from D-fructose by partial purified enzyme. Boronic acid gel separation apparatus was developed to separate them without desalting treatment, suggesting that enzyme reaction and separation process were able to connect for the first time. The purity of D-psicose and D-allose was almost 100%. Furthermore, recovered D-fructose allowed to use enzyme reaction after concentration control. Taking together, we obtain the prospect for the construction of cascade reaction system for rare sugars production.
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