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Elucidation of the biosynthetic mechanisms of chitosan-like bioflocculant and its high production by metabolic engineering

Research Project

Project/Area Number 15K06586
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Hyogo

Principal Investigator

Masahiro Takeo  兵庫県立大学, 工学研究科, 准教授 (40236443)

Co-Investigator(Renkei-kenkyūsha) NEGORO Seiji  兵庫県立大学, 大学院工学研究科, 教授 (00301432)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsbioflocculant / Citrobacter / Metabolic engineering / secretion / memebrane concentration / chitosan / キトサン / 凝集剤 / 代謝工学 / 膜濃縮
Outline of Final Research Achievements

To elucidate the biosynthetic mechanisms of chitosan-like bioflocculant produced by Citrobacter strains and to improve the production, gene addition/disruption studies were performed on genes involved in amino sugar biosynthesis, glucose metabolisms, and polymerization and secretion of the bioflocculant (bfpABCD) in C. freundii IFO 13545. By these trials, the gene modified strains increased the activities, which achieved to 7-times higher activity than that of the wild type strain. To understand the functions of bfpABCD, BfpB, a putative deacetylase, was over-expressed in E. coli and purified. However, the protein showed no deacetylating activity against the bioflocculant. For the recovery of the bioflocculant from the culture supernatants, membrane concentration was also conducted using hollow fiber membrane modules.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (9 results)

  • [Int'l Joint Research] 国立微生物工学研究所(インド)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] カーティン大学(オーストラリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] カーティン大学(マレーシア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] カーティン大学(オーストラリア)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] カーティン大学サラワク校(マレーシア)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Biosynthetic Pathway and Genes of Chitin/Chitosan-Like Bioflocculant in the Genus Citrobacter.2018

    • Author(s)
      Takeo M, Kimura K, Mayilraj S, Inoue T, Tada S, Miyamoto K, Kashiwa M, Ikemoto K, Baranwal P, Kato D, Negoro S.
    • Journal Title

      Polymers (Basel).

      Volume: 10 Issue: 3 Pages: 237-237

    • DOI

      10.3390/polym10030237

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simple ELISA using polyclonal antibody and lectin for determination of soluble chitosan-like polysaccharide in bacterial culture2017

    • Author(s)
      M. Takeo, S. Taguchi, S. Odagaki, P. Baranwal, K. Kimura, S. Negoro
    • Journal Title

      International Journal of BioAnalytical Methods & BioEquivalence Studies

      Volume: 4 Pages: 76-81

    • DOI

      10.19070/2470-4490-170009

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Citrobacter 属細菌の生産するキトサン様バイオ凝集剤の中空糸膜による濃縮2017

    • Author(s)
      武尾正弘、長山尚輝、木村和幸、バランワルプリヤンカ、柏雅美, 加藤太一郎、根来誠司
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Citrobacter 属細菌のキトサン様バイオ凝集剤の生産機構2017

    • Author(s)
      武尾 正弘、木村 和幸、柏 雅美、多田 昇平、井上 卓弥、宮本 弘毅、池本 啓史、根来 誠司
    • Organizer
      第11回日本ゲノム微生物学会年会
    • Place of Presentation
      慶應義塾大学湘南藤沢キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] 糖代謝系遺伝子の破壊によるCitrobacter freundii IFO13545のバイオ凝集剤生産の改善2016

    • Author(s)
      柏 雅美、宮本 弘毅、木村 和幸、池本 啓史、根来 誠司、武尾 正弘
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Related Report
      2016 Research-status Report
  • [Presentation] Citrobacter 属細菌におけるキトサン様バイオ凝集剤の多糖化・分泌関連遺伝子の探索2016

    • Author(s)
      武尾 正弘、池本 啓史、宮本 弘毅、木村 和幸、柏 雅美、加藤 太一郎、根来 誠司
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Related Report
      2016 Research-status Report
  • [Presentation] 放射性物質やその吸着剤残渣を除去するバイオ凝集剤の生産2016

    • Author(s)
      武尾 正弘、柏 雅美、西岡 洋
    • Organizer
      国際フロンティア産業メッセ2016
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2016 Research-status Report
  • [Presentation] バイオ凝集剤生産菌Citrobacter freundii IFO13545株の定量PCRによる遺伝子発現解析2015

    • Author(s)
      池本啓史、柏雅美、木村和幸、根来誠司、武尾正弘
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル、鹿児島県鹿児島市
    • Year and Date
      2015-10-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Citrobacter freundii IFO13545遺伝子破壊株によるグルコースからのバイオ凝集剤生産の改善2015

    • Author(s)
      柏雅美、宮本弘毅、木村和幸、多田昇平、根来誠司、武尾正弘
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル、鹿児島県鹿児島市
    • Year and Date
      2015-10-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 培養液中のキトサン様多糖のELISAによる定量2015

    • Author(s)
      武尾正弘、田口冴恵、柏雅美、木村和幸、根来誠司
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル、鹿児島県鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
  • [Presentation] トバモライト系吸着材とバイオ凝集剤の併用による汚染土壌等からの放射性セシウムの除去2015

    • Author(s)
      柏雅美、姫路佳孝、西岡 洋、武尾正弘
    • Organizer
      環境技術学会第15回年次大会
    • Place of Presentation
      大阪産業大学、大阪府大東市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-12-27  

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