Elucidation of the biosynthetic mechanisms of chitosan-like bioflocculant and its high production by metabolic engineering
| Project/Area Number |
15K06586
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Hyogo |
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Principal Investigator |
Masahiro Takeo 兵庫県立大学, 工学研究科, 准教授 (40236443)
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| Co-Investigator(Renkei-kenkyūsha) |
NEGORO Seiji 兵庫県立大学, 大学院工学研究科, 教授 (00301432)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | bioflocculant / Citrobacter / Metabolic engineering / secretion / memebrane concentration / chitosan / キトサン / 凝集剤 / 代謝工学 / 膜濃縮 |
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| Outline of Final Research Achievements |
To elucidate the biosynthetic mechanisms of chitosan-like bioflocculant produced by Citrobacter strains and to improve the production, gene addition/disruption studies were performed on genes involved in amino sugar biosynthesis, glucose metabolisms, and polymerization and secretion of the bioflocculant (bfpABCD) in C. freundii IFO 13545. By these trials, the gene modified strains increased the activities, which achieved to 7-times higher activity than that of the wild type strain. To understand the functions of bfpABCD, BfpB, a putative deacetylase, was over-expressed in E. coli and purified. However, the protein showed no deacetylating activity against the bioflocculant. For the recovery of the bioflocculant from the culture supernatants, membrane concentration was also conducted using hollow fiber membrane modules.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Biosynthetic Pathway and Genes of Chitin/Chitosan-Like Bioflocculant in the Genus Citrobacter.2018
Author(s)
Takeo M, Kimura K, Mayilraj S, Inoue T, Tada S, Miyamoto K, Kashiwa M, Ikemoto K, Baranwal P, Kato D, Negoro S.
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Journal Title
Polymers (Basel).
Volume: 10
Issue: 3
Pages: 237-237
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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