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Developments of fluorescent molecular probes for the simultaneous detections of various neurotransmitters and application to medical diagnosis

Research Project

Project/Area Number 15K06592
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Suzuki Yoshio  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (60321907)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords蛍光 / センサー / 分子プローブ / 神経伝達物質 / 分子設計
Outline of Final Research Achievements

Fluorescent reagents, which have transition metal complex as recognition site for dopamine, peptides as recognition site of oxytocin and fluorophore such as cyanopyranyl moiety have been developed for the selective detection of neurotransmitters. These compounds indicated weak fluorescence, whereas strong emission was observed after addition of target neurotransmitters. The increases of fluorescence intensities were concentration-dependent, from which a good linear relationships were observed by plotting the data as a function of neurotransmitters concentrations. The bindings and detections of fluorescent probes with target neurotransmitters were not affected by the presence of foreign substances, thereby allowing for the highly selective detection of neurotransmitters. These fluorescent probes can be broadly employed in detection protocols of neurotransmitters by the scientific community in diverse areas of research.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、開発した分析試薬を用いることにより、これまで計測が困難であった神経伝達物質の高感度かつ高選択的検出が可能になったという分析化学分野の発展に繋がる学術的に意義のある成果である。さらに、神経伝達物質の動的挙動をリアルタイムで計測出来る技術にも繋がり、現在根本的な治療法が確立していない神経変性疾患に対する理解が深まる可能性を秘めている。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Development of a fluorescent peptide for the highly sensitive and selective detection of vascular endothelial growth factor2018

    • Author(s)
      Yoshio Suzuki
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 276 Pages: 230-237

    • DOI

      10.1016/j.snb.2018.08.108

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amplified Zinc Signal at a Nanocarbon Film Electrode for Lipopolysaccharide Detection2018

    • Author(s)
      Dai Kato, Yoshio Suzuki, Kyoko Yoshioka, Tomoyuki Kamata, Masami Todokoro, and Osamu Niwa
    • Journal Title

      ACS Applied Nano Materials

      Volume: 1 Issue: 10 Pages: 5425-5429

    • DOI

      10.1021/acsanm.8b01663

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a fluorescent peptide for the highly sensitive and selective detection of oxytocin2018

    • Author(s)
      Suzuki Yoshio
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 254 Pages: 321-328

    • DOI

      10.1016/j.snb.2017.07.080

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 総タンパク質の定量法2018

    • Author(s)
      鈴木 祥夫
    • Journal Title

      ぶんせき

      Volume: 517 Pages: 2-9

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生体物質を分光学的に検出するための機能性材料の創製2017

    • Author(s)
      鈴木 祥夫
    • Journal Title

      化学工業

      Volume: 68 Pages: 47-54

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Design and Synthesis of Fluorescent Reagents for Selective Detection of Dopamine2017

    • Author(s)
      Yoshio Suzuki
    • Journal Title

      SENSORS AND ACTUATORS B-CHEMICAL

      Volume: 239 Pages: 383-389

    • DOI

      10.1016/j.snb.2016.08.019

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of fluorescent probes for “On-Off” switching based detection of lectin-saccharide interactions2015

    • Author(s)
      Yoshio Suzuki, Atsushi Kuno, Yasunori Chiba
    • Journal Title

      Sensors and Actuators B: Chemical

      Volume: 220 Pages: 389-397

    • DOI

      10.1016/j.snb.2015.05.041

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances2015

    • Author(s)
      Yoshio Suzuki, Kenji Yokoyama
    • Journal Title

      Biosensors

      Volume: 5 Issue: 2 Pages: 337-363

    • DOI

      10.3390/bios5020337

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 神経伝達物質を高感度検出するための新規蛍光分子プローブの創製2018

    • Author(s)
      鈴木 祥夫
    • Organizer
      第16回ホスト-ゲスト・超分子化学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Developments of Novel Fluorescent Molecular Probes for the Selective Detection of Neurotransmitters2018

    • Author(s)
      鈴木 祥夫
    • Organizer
      ACS National Meeting & Expo
    • Related Report
      2018 Annual Research Report
  • [Presentation] Developments of Fluorescent Reagents for the Selective Detection of Neurotransmitters2018

    • Author(s)
      鈴木 祥夫
    • Organizer
      BIT's 9th World Gene Convention-2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 神経伝達物質を選択的に検出するための新規蛍光分子プローブの開発2017

    • Author(s)
      鈴木 祥夫
    • Organizer
      日本化学会 第97春季年会
    • Place of Presentation
      慶應義塾大学 日吉キャンパス(神奈川県・横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Development of Fluorescent Molecular Probes based on Cyanopyranyl Fluorophore for the Detection of Biological Substances such as Glycoconjugates or Dopamine2017

    • Author(s)
      Yoshio Suzuki
    • Organizer
      Pittcon 2017
    • Place of Presentation
      シカゴ(アメリカ)
    • Year and Date
      2017-03-05
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 神経伝達物質を選択的に検出するための新規蛍光分析試薬の 創製2017

    • Author(s)
      鈴木 祥夫
    • Organizer
      第15回ホストーゲスト・超分子化学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 神経伝達物質を選択的に検出するための新規蛍光分析試薬の開発2017

    • Author(s)
      鈴木 祥夫、田中 睦生
    • Organizer
      第77回分析化学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 神経伝達物質を選択的に検出するための新規蛍光分子プローブの創製2017

    • Author(s)
      鈴木 祥夫、田中 睦生
    • Organizer
      2017年光化学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Developments of Fluorescent Reagents for the Selective Detection of Neurotransmitters2017

    • Author(s)
      Yoshio Suzuki
    • Organizer
      2017 RACI Centenary Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of Fluorescent Molecular Probes for the Highly Sensitive Detection of Proteins and Glycoconjugates2017

    • Author(s)
      Yoshio Suzuki
    • Organizer
      BIT's 8th World Gene Convention-2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ドーパミンを選択的に検出するための新規蛍光試薬の開発2016

    • Author(s)
      鈴木 祥夫
    • Organizer
      第10回バイオ関連化学シンポジウム
    • Place of Presentation
      石川県立音楽堂・もてなしドーム地下イベント広場(石川県・金沢市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] ドーパミンを選択的に検出するための新規蛍光物質の創製2016

    • Author(s)
      鈴木 祥夫
    • Organizer
      第14回ホスト・ゲスト化学シンポジウム
    • Place of Presentation
      高知城ホール(高知県・高知市)
    • Year and Date
      2016-06-04
    • Related Report
      2016 Research-status Report
  • [Presentation] Development of Fluorescent Magnetic Particles for “On-Off” Switching based Detection of Various Lectin-Saccharide Interactions2016

    • Author(s)
      Yoshio Suzuki
    • Organizer
      BIT’s 9th Annual World Protein & Peptide Conference
    • Place of Presentation
      大連(中国)
    • Year and Date
      2016-04-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ドーパミンを選択的に検出するための新規蛍光分子プローブの開発2016

    • Author(s)
      鈴木 祥夫、長坂 和明、高島 一郎、山本 慎也
    • Organizer
      日本化学会 第96春季年会 (2016)
    • Place of Presentation
      同志社大学 京田辺キャンパス
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Book] 材料表面の親水・親油の評価と制御設計2016

    • Author(s)
      鈴木 祥夫
    • Total Pages
      600
    • Publisher
      株式会社テクノシステム
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 生体分子検出用蛍光物質2017

    • Inventor(s)
      鈴木 祥夫
    • Industrial Property Rights Holder
      鈴木 祥夫
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-039176
    • Filing Date
      2017-03-02
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] ドーパミン検出用蛍光物質2015

    • Inventor(s)
      鈴木祥夫、山本慎也、長坂和明、高島一郎
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-233571
    • Filing Date
      2015-11-30
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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