Elucidation of neuronal networks to control conflicts between different memories

• Project/Area Number: 15K06698
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo
• Principal Investigator: Matsushima Ayano 東京大学, 大学院医学系研究科(医学部), 客員研究員 (10706740)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: E-SARE / 活動依存的プロモータ / アデノ関連ウイルス / DNA組み換え / DNA組み替え

Outline of Final Research Achievements

In this study, we modified already-existing activity-dependent promoter to dissect active and inactive subpopulations within a neuronal population defined by a promoter. By combining the modified activity-dependent promoter and Boolean logical operation using multiple recombinases, we succeeded in manipulating their activities in opposite direction.
Here, we applied this method to CA1 region of PV-Cre mice. Activity-dependent division of PV+ neurons revealed the existence of endogenous activity gradient that is programmed developmentally, and this gradient contributes to the coherent locomotion on the environmental geometry.

Research Products

• [Presentation] Postsynaptic mechanisms underlying activity-dependent adaptation of glutamatergic synapses. (2016)
  • Author(s): Bito H, Kim R, Inoue M, Nonaka M, Ishii Y, Sakai K, Kawashima T, Yagishita-Kyo N, Matsushima A, Kamijo S, Goto M, Kobari S, Okamura M, Endo T, Horigane S, Okuno H, Takemoto-Kimura S, Fujii H.
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22