Optical inactivation of GluA1 homomeric receptors in a single dendritic spine.
| Project/Area Number |
15K06713
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 学習記憶 / 光操作 / AMPA受容体 |
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| Outline of Final Research Achievements |
AMPA type glutamate receptors (AMPA-Rs) are known to have the important roles in synaptic activities and animal learning in vivo. AMPA-Rs are composed of variable combinations of four subunits, GluA1-4. In this project, to show the specificity of our GluA1 CALI technology, we found that CALI with Z9139 antibody showed the complex specificity to GluA1 homomer. We also elucidate the molecular specificity fo CALI with Z9139 in vivo using GluA1 KO mice. These results were published in Nature Biotechnology. We now conduct the CALI in singe spine on primary cultured neurons and acute hippocampal slices to elucidate the spatio-temporal function of GluA1 homomer.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Social isolation suppresses actin dynamics and synaptic plasticity through ADF/cofilin inactivation in the developing rat barrel cortex2017
Author(s)
Tada H, Miyazaki T, Takemoto K, Jitsuki S, Nakajima W, Koide M, Yamamoto N, Taguchi A, Kawai H, Komiya K, Suyama K, Abe H, Sano A, Takahashi T.
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Journal Title
Sci. Rep
Volume: 7
Issue: 1
Pages: 8471-8471
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neonatal isolation augments social dominance by altering actin dynamics in the medial prefrontal cortex.2016
Author(s)
Tada H, Miyazaki T, Takemoto K, Takase K, Jitsuki S, Nakajima W, Koide M, Yamamoto N, Komiya K, Suyama K, Sano A, Taguchi A, Takahashi T
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Journal Title
Proc Natl Acad Sci U S A
Volume: 113
Issue: 45
DOI
Related Report
Peer Reviewed / Open Access
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