| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
AMPA type glutamate receptors (AMPA-Rs) are known to have the important roles in synaptic activities and animal learning in vivo. AMPA-Rs are composed of variable combinations of four subunits, GluA1-4. In this project, to show the specificity of our GluA1 CALI technology, we found that CALI with Z9139 antibody showed the complex specificity to GluA1 homomer. We also elucidate the molecular specificity fo CALI with Z9139 in vivo using GluA1 KO mice. These results were published in Nature Biotechnology. We now conduct the CALI in singe spine on primary cultured neurons and acute hippocampal slices to elucidate the spatio-temporal function of GluA1 homomer.
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