| Project/Area Number |
15K06728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kyoto University (2017)
Institute of Physical and Chemical Research (2015-2016) |
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Principal Investigator |
Goto Akihiro 京都大学, 医学研究科, 特定助教 (10741332)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | cofilin / LTP / SuperNova / CALI / Cofilin / 光操作 / 記憶の消去 |
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| Outline of Final Research Achievements |
We previously found that in the initial phase of LTP, cofilin is transported to the spine, forms a stable complex with F-actin, persistently accumulates at the spine, and consolidates spine expansion. To spatiotemporally regulate cofilin activity during LTP, we introduced CALI (chromophore-assisted light inactivation) system, a technique to inactivate target proteins with light irradiation through reactive oxygen. For this purpose, we generated a fusion protein between cofilin and SuperNova (SN), a protein of GFP family that generates reactive oxygen upon light illumination.
We showed cofilin is critical for maintenance of LTP up to 40 min after induction, and the inactivation of cofilin had negligible effect on LTP induction and the volume of unstimulated spine .
Memory was significantly impaired when 593 nm was irradiated on CA1 neurons expressing CFL-SN after memory task.
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