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Structural Analysis of glial cells by using high resolution imaging method

Research Project

Project/Area Number 15K06734
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionThe University of Tokyo

Principal Investigator

Iwasaki Hirohide  東京大学, 大学院医学系研究科(医学部), 講師 (30342752)

Co-Investigator(Renkei-kenkyūsha) OKABE Shigeo  東京大学, 大学院医学系研究科, 教授 (60204012)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsグリア細胞 / 微細形態解析 / 電子顕微鏡 / 光学顕微鏡 / ミクログリア / FIB-SEM / 神経科学 / 解剖学
Outline of Final Research Achievements

In the central nervous system of the mammalian brain, three types of glial cells have been identified; astrocyte, microglia and oligodendrocyte. Among them, oligodendrocyte forms myelin around axons. Other types of glial cells might contribute to the synaptic functions of the brain by affecting the synapse formation/elimination.
In this research, I analyzed the precise structure of astrocyte and microglia by using CLEM (Correlative anaylysis of light- and electron- microscopy) method. Particularly, I succeeded the comprehensive three-dimensional reconstruct of the fine protrusions of single microglia.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Near-infrared deep brain stimulation via upconversion nanoparticle-mediated optogenetics.2018

    • Author(s)
      Chen S, Weitemier AZ, Zeng X, He L, Wang X, Tao Y, Huang AJY, Hashimotodani Y, Kano M, Iwasaki H, Parajuli LK, Okabe S, Teh DBL, All AH, Tsutsui-Kimura I, Tanaka KF, Liu X, McHugh TJ.
    • Journal Title

      Science

      Volume: 359 Issue: 6376 Pages: 679-684

    • DOI

      10.1126/science.aaq1144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap12017

    • Author(s)
      Cornelia Koeberle Solveigh、Tanaka Shinji、Kuriu Toshihiko、Iwasaki Hirohide、Koeberle Andreas、Schulz Alexander、Helbing Dario-Lucas、Yamagata Yoko、Morrison Helen、Okabe Shigeo
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 13409-13409

    • DOI

      10.1038/s41598-017-13728-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diversity of the molecular mechanisms of synapse formation in the central nervous system2017

    • Author(s)
      Risa Iguchi, Hirohide Iwasaki, Shigeo Okabe
    • Journal Title

      Seikagaku

      Volume: 89 Pages: 77-80

    • NAID

      40021119778

    • Related Report
      2017 Annual Research Report
  • [Presentation] Three-dimensional reconstruction of dendritic spines by scanning electron microscopy2018

    • Author(s)
      岩﨑広英、岡部繁男
    • Organizer
      日本解剖学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Three-dimensional - reconstruction of dendritic spines using scanning electron microscopy2017

    • Author(s)
      Hirohide Iwasaki, Shigeo Okabe
    • Organizer
      The 3rd East-Asia Microscopy Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of techniques for correlated imaging of in vivo two-photon microscopy and electron microscopy2016

    • Author(s)
      岩崎広英、田中慎二、岡部繁男
    • Organizer
      日本解剖学会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Three-Dimensional Reconstruction of Neural Tissue from Seiral Sections Collected by ATUM2015

    • Author(s)
      Hirohide Iwasaki, Shigeo Okabe
    • Organizer
      East Asia Microscopy Conference
    • Place of Presentation
      姫路商工会議所(兵庫県姫路市)
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ATUMを用いた連続切片からのSEMによる神経 組織の立体再構築2015

    • Author(s)
      岩崎広英、岡部繁男
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2015-05-13
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] Dendrites: Development and Disease2016

    • Author(s)
      Hirohide Iwasaki, Shinji Tanaka and Shigeo Okabe
    • Total Pages
      608
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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