| Project/Area Number |
15K06741
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Nerve anatomy/Neuropathology
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
本間 俊作 福島県立医科大学, 医学部, 准教授 (20261795)
向笠 勝貴 福島県立医科大学, 医学部, 助教 (60706349)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | アポトーシス / 運動神経 / Foxp1 / Hox / 鳥類 / 頚髄 / 外側運動柱 / 細胞死 / 発生 / FoxP1 / ニワトリ / Ｆｏｘｐ１ / Ｈｏｘ / ＨｏｘＣ６ / Ｂｃｌ２ / Raldh2 / Lhx1 / microRNA-9 / 僧帽筋 |
|---|
| Outline of Final Research Achievements |
In the avian embryo, a certain population of motor neurons (MNs) in the non-limb-innervating cervical spinal cord undergoes apoptosis in early developmental stages. We found that these apoptotic MNs can be distinguished by Foxp1 expression. After apoptosis inhibition Foxp1+ MNs exhibited characteristics of lateral motor column (LMC) MNs that innervate limb muscles. Functional analyses of Foxp1 indicated a role for Foxp1 in specifying LMC fate, but not in apoptosis induction. Furthermore, ectopic expression of Hox genes that are normally expressed in the brachial region prevented apoptosis, and directed Foxp1+ MNs to LMC MNs at the cervical level. These results suggest that Foxp1+ MNs in the cervical spinal cord are homologous to LMC MNs, and are eliminated by apoptosis depending on the Hox expression pattern.
|
