Targeted astrocytic functional modification using optogenetics - toward realization of artificial control of the basal ganglia circuit
| Project/Area Number |
15K06743
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Nara Medical University |
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Principal Investigator |
Tatsumi Kouko 奈良県立医科大学, 医学部, 准教授 (90208033)
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| Co-Investigator(Kenkyū-buntansha) |
森田 晶子 奈良県立医科大学, 医学部, 助教 (70647049)
和中 明生 奈良県立医科大学, 医学部, 教授 (90210989)
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| Co-Investigator(Renkei-kenkyūsha) |
NANBU Atsushi 生理学研究所, システム脳科学研究領域・生体システム研究部門, 教授 (80180553)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | Olig2 / アストロサイト / GFAP / DREADDシステム / 淡蒼球 / DREADD / ムスカリン型アセチルコリン受容体 / 大脳基底核 / パーキンソン病モデルマウス / オプトジェネティクス / アデノ随伴ウイルス / 光遺伝学 |
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| Outline of Final Research Achievements |
We found Olig2-lineage mature astrocytes (Olig2-AS) in the adult brain using Olig2CreER;YFP transgenic mice. They were found to be preferentially clustered in some adult brain nuclei. We made distribution map of Olig2-AS in the adult mouse brain, and compared the map with the distribution of GFAP-positive astrocytes (GFAP-AS) visualized in GFAPCre;YFP mice. Brain regions rich in Olig2-AS tended to lack GFAP-AS. Even within a single brain nucleus, Olig2-AS and GFAP-AS frequently occupied mutually exclusive territories. These findings strongly suggest that Olig2-AS is a subpopulation of astrocytes and that it differs from GFAP-AS in its distribution pattern and perhaps also in its function. Immunoelectron microscopic study showed that fine processes of Olig2-AS are preferentially associated with GABAergic terminals, suggesting they are involved in inhibitory neuronal transmission. We activated Olig2-AS in the globus pallidus by DEADD method, and observe the influence to neural activity.
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Report
(4 results)
Research Products
(11 results)
