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Molecular mechanism of Reelin-induced neuronal aggregation in the developing mouse cereberal cortex

Research Project

Project/Area Number 15K06745
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKeio University

Principal Investigator

Hayashi Kanehiro  慶應義塾大学, 医学部(信濃町), 講師 (60373354)

Research Collaborator Inoue Seika  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発生 / 大脳新皮質 / 層構造 / 細胞凝集 / 細胞接着 / Reelin / N-cadherin / 神経細胞凝集
Outline of Final Research Achievements

The mammalian neocortex has the six layered structure organized by excitatory neurons, which enables higher brain functions. A secretary molecule ‘Reelin’ is essential for the layer formation, but the molecular mechanism based on Reelin for the layer formation remains to be elucidated. The purpose of the current project is to clarify cellular and molecular mechanisms to form the layered structure based on Reelin during cortical development.
In this study, we found that Reelin-inducible neuronal aggregate was mediated by N-cadherin-based cellular adhesion and that this promotion of cellular adhesion by Reelin was in a transient manner. Moreover, we revealed p120 catenin and Nck were involved in this N-cadherin-based cellular adhesion. These results were presented in several scientific meetings and published in an international journal.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (15 results) (of which Int'l Joint Research: 3 results) Book (1 results)

  • [Journal Article] Reelin transiently promotes N-cadherin-dependent neuronal adhesion during mouse cortical development2017

    • Author(s)
      Yuki Matsunaga, Mariko Noda, Hideki Murakawa, Kanehiro Hayashi, Arata Nagasaka, Seika Inoue, Takaki Miyata, Takashi Miura, Ken-ichiro Kubo, and Kazunori Nakajima
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 114 Issue: 8 Pages: 2048-2053

    • DOI

      10.1073/pnas.1615215114

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The brain-specific RasGEF very-KIND is required for normal dendritic growth in cerebellar granule cells and proper motor coordination2017

    • Author(s)
      Hayashi K, Furuya A, Sakamaki Y, Akagi T, Shinoda Y, Sadakata T, Hashikawa T, Shimizu K, Minami H, Sano Y, Nakayama M, and Furuichi T.
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 3 Pages: e0173175-e0173175

    • DOI

      10.1371/journal.pone.0173175

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cellular dynamics of neuronal migration in the hippocampus.2015

    • Author(s)
      Hayashi K, Kubo K, Kitazawa A and Nakajima K
    • Journal Title

      Front.Neurosci

      Volume: in press Pages: 135-135

    • DOI

      10.3389/fnins.2015.00135

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The COUP-TFII/Neuropilin-2 is a molecular switch steering diencephalon-derived GABAergic neurons in the developing mouse brain2015

    • Author(s)
      Shigeaki Kanatani, Takao Honda, Michihiko Aramaki, Kanehiro Hayashi, Ken-ichiro Kubo, Mami Ishida, Daisuke H. Tanaka, Takeshi Kawauchi, Katsutoshi Sekine, Sayaka Kusuzawa, Takahiko Kawasaki, Tatsumi Hirata, Hidenori Tabata, Per Uhlen and Kazunori Nakajima
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 112(36) Issue: 36

    • DOI

      10.1073/pnas.1420701112

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Structural analyses of radial glial fibers in the developing reeler neocortex2017

    • Author(s)
      Shin MK, Kitazawa A, Matsunaga Y, Hayashi K, Kubo KI, and Nakajima K.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The Role of Nck as a downstream effector of the Reelin signaling2017

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第60回日本神経化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular mechanisms of neuronal aggregation caused by Reelin in the developing mouse neocortex2017

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第60回日本神経化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Region-dependent differences in the migratory capacity of hippocampal CA1 and neocortical neurons during brain development2017

    • Author(s)
      北澤彩子、シン ミンギョン、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第60回日本神経化学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス大脳新皮質形成におけるreelin-Nck2シグナルの役割2017

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第40回日本分子生物学会年会・第90回日本生化学会大会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular mechanisms of Reelin-induced neuronal aggregation in the developing mouse neocortex2017

    • Author(s)
      Inoue S, Hayashi K, Kubo KI, and Nakajima K
    • Organizer
      18th International Congress of Developmental Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of the molecular mechanisms underlying Reelin-induced neuronal aggregation in the mouse neocortex2016

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      2016 The American Society for Cell Biology (ASCB) Annual Meeting
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Morphological analyses of radial glial cells in the developing mouse neocortex2016

    • Author(s)
      シン・ミンギョン、北澤彩子、松永友貴、林周宏、久保健一郎、仲嶋一範
    • Organizer
      Society for Neuroscience, Neuroscience 2016 meeting
    • Place of Presentation
      サンディエゴ(アメリカ)
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of Reelin-Nck signaling in mouse neocortex2016

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第38回日本生物学的精神医学会・第59回日本神経化学会大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 大脳新皮質においてリーリン分子により誘導される神経細胞凝集を担うシグナル伝達経路の探索”2016

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第38回日本生物学的精神医学会・第59回日本神経化学会大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス大脳新皮質発生におけるReelin-Nckシグナル経路の役割2016

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 大脳新皮質においてリーリン分子により引き起こされる神経細胞凝集のシグナル伝達経路の探索2016

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Function of the Reelin-Nck signaling pathway during mouse neocortical development2016

    • Author(s)
      林周宏、井上聖香、久保健一郎、仲嶋一範
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Investigation of the molecular mechanisms that regulate Reelin-induced neuronal aggregation in the mouse neocortex2016

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of the molecular mechanisms that underlie Reelin-induced neuronal aggregation in the mouse neocortex2016

    • Author(s)
      井上聖香、林周宏、久保健一郎、仲嶋一範
    • Organizer
      第9回神経発生討論会・難治疾患共同研究拠点共同開催学術集会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Book] Encyclopedia of Signaling Molecules second edition 'Reelin'2018

    • Author(s)
      Hayashi K, Inoue S, Nakajima K
    • Publisher
      Springer International Publishing
    • ISBN
      9783319672007
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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