| Project/Area Number |
15K06760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Keio University |
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Principal Investigator |
Tomita Yutaka 慶應義塾大学, 医学部(信濃町), 講師(非常勤) (60276251)
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| Research Collaborator |
SUZUKI Norihiro
KANNO Iwao
UNEKAWA Miyuki
MASAMOTO Kazuto
YUKI Hiroya
SOGA Naoto
WATANABE Tatsushi
HATAKEYAMA Nao
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | マイクログリア / neurovascular unit / 脳梗塞 / 二光子顕微鏡 / 微小循環 / 低酸素負荷 / 血管新生 |
|---|
| Outline of Final Research Achievements |
In genetically-modified mice (CX3CR1-GFP mice）expressing the cortical microglia with green fluorescence protein (GFP), microglia and cortical microvessels were imaged through a closed-cranial window by using a Tomita-Seylaz method with two-photon laser scanning fluorescence microscopy repeatedly up to 3 weeks. There were no detectable differences in the soma area, number density, and number of processes of the GFP-positive microglia, whereas migrated and newborn microglia could be detected. Furthermore, appearance and/or migration of microglia adjacent to neovascular capillary under chronic hypoxia (8% oxygen) was exhibited.
A stimulation with a blue light in mice genetically expressing a light-sensitive cation channel, channelrhodopsin-2 in cortical astrocytes or neurons elicited intensity-dependent increase of cerebral blood flow. Pharmacological study exhibited independent mechanism of CBF control.
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