| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Extracellular acidic pH of ~6.5 has been shown to take place with ischemia and neurodegenerative. However, the mechanisms underlying acidic pH-induced actions are poorly understood. Proton-sensing OGR1 family G-protein coupled receptors (GPCRs, including OGR1, GPR4, and TDAG8) have been shown to be expressed in brain. This study has focused on the roles of proton-sensing GPCRs in the brain injury in a mouse ischemia reperfusion model. We also analyzed roles of proton-sensing GPCRs in mouse microglia and neuronal cells in acidic pH medium. Our results suggested that acidic pH /TDAG8 functions protectively for cerebral infarction by the mouse ischemia model possibly due to the mechanism involving inhibitory actions against microglia function. And also we showed the involvement of OGR1 and/or GPR4 in the acidic pH regulation of neuronal cell functions. Thus, the elucidation of acidic pH actions may provide therapeutic targets of neurodegenerative disorders such as ischemic stroke.
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