| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We analyzed the expression and functions of human HES factors that play a central role in the maintenance of neural stem cells (NSCs) and revealed the activities of HES1/4/5 to inhibit neuronal differentiation and maintain NSCs when overexpressed. And we found that both HES1 and HES4 are expressed in the ventricular zone of brains of cynomolgus monkey embryos. The transgenic mice, in which Hes is overexpressed in NSCs of brains by using the Tet-ON system, showed a phenotype of expanded ventricles with thinner cortical plate, due to the maintenance of NSCs and inhibition of neuronal differentiation. In the Hes1-overexpressing Tg mice, basal radial glia-like cells increased in number, suggesting that the alteration in Hes expression levels contributes to the mammalian brain evolution.
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