| Project/Area Number |
15K06802
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Hayashi Toshinori 鳥取大学, 医学部, 准教授 (60580925)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ネッタイツメガエル / アフリカツメガエル / イベリアトゲイモリ / ゲノム編集 / ノックイン / トランスジェニック / 両生類 / 再生 / 変態 / ノックアウト |
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| Outline of Final Research Achievements |
Following completion of the genome sequences of amphibians, gene targeting techniques have become increasingly important for the further development of amphibian research in the life sciences. Recent advances in genome editing using the CRISPR-Cas system now allow for reverse genetics in the amphibians, Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl. In order to refine these amphibians as model animals for life science research, we tried to develop a highly efficient and rapid workflow for gene knock-out, gene knock-in, conditional knock-out, and safe harbor transgenic systems.
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