Expression analysis of DoK-1, Dok-2 and Dok-3 genes in mice expressing Tax, a human T-cell leukemia virus type 1 oncoprotein
Project/Area Number |
15K06806
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
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Research Institution | Rakuno Gakuen University |
Principal Investigator |
Ohsugi Takeo 酪農学園大学, 獣医学群, 教授 (00211102)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | HTLV-1 / Tax / 成人T細胞白血病 / Dok-1 / Dok-2 / Dok-3 / 遺伝子改変マウス / DOK1 / DOK2 / DOK3 / DOK1 / Dok1 / Dok2 / Dok3 |
Outline of Final Research Achievements |
Human T-cell leukemia virus type 1 (HTLV-1) can cause adult T-cell leukemia/lymphoma (ATL). The Tax protein encoded by HTLV-1 appears to be a key element in the early stage of ATL development. We examined the expression of the downstream of tyrosine kinase (Dok) family members Dok-1, Dok-2 and Dok-3, recently reported to be tumor suppressors, in HTLV-1-infected T cells and transgenic mice expressing the tax gene. Dok-2 and Dok-3 expression was significantly reduced in HTLV-1-infected T cells compared with their expression in uninfected T cells, and the expression of Dok-3 was reduced by the induction of Tax expression in T cells. The transgenic mice showed low expression of Dok-1, Dok-2 and Dok-3 in mice with T cell leukemia. Mice showed low Dok-2 expression at 5-6 months of age before disease onset. These results suggest that downregulation of Dok-2 and Dok-3 by the expression of the viral tax gene is the first step in the development of ATL.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Evaluation of a combination of alfaxalone with medetomidine and butorphanol for inducing surgical anesthesia in laboratory mice2016
Author(s)
Higuchi, S., Yamada, R., Hashimoto, A., Miyoshi, K.,Yamashita, K. and Ohsugi, T.
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Journal Title
Japanese Journal of Veterinary Research
Volume: 64
Issue: 2
Pages: 131-139
DOI
NAID
ISSN
0047-1917
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Development and molecular analysis of synthetic lethality by targeting EZH1/2 in ATL and HTLV-1-infected cells.2017
Author(s)
Yamagishi, M., Hori, M., Fujiwara, D., Honma, D., Adachi, N., Ohsugi, T., Nakano, K., Nakashima, M., Kobayashi, S., Iwanaga, M., Utsunomiya, A., Tanaka, Y., Okada, S., Tsukasaki, K., Toninai, K., Araki, K., Watanabe, T. and Uchimaru, K.
Organizer
18th International Conference on Human Retrovirology HTLV and Related Viruses
Place of Presentation
ホテルグランドアーク半蔵門(東京都)
Year and Date
2017-03-07
Related Report
Int'l Joint Research
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[Presentation] Tamoxifen inhibits the infiltration of human T-cell leukemia virus type 1-infected cells into organs in a mouse model of adult T-cell leukemia.2017
Author(s)
Tanaka, Y., Kozuka, T., Yamagishi, M., Hirose, T., Nagano, Y., Nishii, R., Kitamura, H., Takehana, K., Watanabe, T. and Ohsugi, T.
Organizer
18th International Conference on Human Retrovirology HTLV and Related Viruses
Place of Presentation
ホテルグランドアーク半蔵門(東京都)
Year and Date
2017-03-07
Related Report
Int'l Joint Research
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