Analysis of Inv gene functions by generation of novel transgenic mice.

Research Project

Project/Area Number	15K06808
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Laboratory animal science
Research Institution	Kitasato University
Principal Investigator	Watanabe Daisuke 北里大学, 理学部, 講師 (00260175)
Research Collaborator	MENO Chikara
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	Inv遺伝子 / 左右軸形成 / 多発性嚢胞腎 / コンディショナルノックアウト / コンディシショナルレスキュー / トランスジェニックマウス / Inv 遺伝子 / コンデショナルノックアウトマウス / コンディショナルレスキューマウス / inv遺伝子 / コンディショナルノックアウトマウス / 繊毛
Outline of Final Research Achievements	The Inv gene is an important gene involved in the left/ right axis determination in the development of animals. It is also known to be a causative gene of nephronophthisis type 2 (NPHP2)in human. In this study, to analyze the physiological function of the Inv gene, we devised a conditional rescue technic to induce specific genes inactivation in arbitrary tissues and we succeeded in making conditional rescue mice capable of Inv gene inactivation in tissue-dependent manner. The transgenic mice produced in this study will be used for elucidating the mechanism of the left/right axis determination of animals and the basic research for treating human kidney disease. In addition, the conditional rescue technic developed in this study is expected to be widely applied as a new analytical method of gene function in the future.