Integrating multiple omics analysis for understanding the pathological mechanism of chronic hepatitis B virus infection and identifying potential molecular targets using humanized chimeric mice

• Project/Area Number: 15K06810
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Jikei University School of Medicine
• Principal Investigator: TSUBOTA Akihito 東京慈恵会医科大学, 医学部, 教授 (90322643)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: B型肝炎 / B型肝炎ウイルス / ヒト化肝臓キメラマウス / トランスクリプトーム解析

Outline of Final Research Achievements

Nucleos(t)ide analogues, the standard of care for chronic hepatitis B virus (HBV) infection, cannot eliminate the virus completely from HBV-infected hepatocytes. Therefore, novel agents with different antiviral actions are needed. We have established a humanized liver-chimeric mouse model at a relatively low cost. Using this model, we performed comprehensive transcriptome analyses of liver tissues from the hyperacute phase to the chronic infection phase. Surprisingly, there was a much higher number of weakly expressed mRNAs than of strongly expressed mRNAs. Three miRNAs were consistently upregulated during this period. Inhibitors of these miRNAs reduced the efficiency of HBV infection and replication. Some mRNAs that specifically interacted with these miRNAs affected the chronicity of the infection. Proteomic and metabolomic analyses showed elevated lipoprotein-related concentrations. HBV is suggested to adapt host circumstances by stealth nature against the host innate immune system.

Research Products

• [Journal Article] Efficacy and safety of ledipasvir/sofosbuvir for genotype 1b chronic hepatitis C patients with moderate renal impairment (2018)
  • Author(s): Okubo T, Atsukawa M, Tsubota A, Toyoda H, Shimada N, Abe H, Kato K, Hayama K, Arai T, Nakagawa-Iwashita A, Itokawa N, Kondo C, Kawamoto C, Iio E, Tanaka Y, Kumada T, Iwakiri K.
  • Journal Title: Hepatol Int Volume: 12 Issue: 2 Pages: 133-142
  • DOI: 10.1007/s12072-018-9859-9
  • Peer Reviewed
• [Journal Article] Hepatocellular adenoma in a woman who was undergoing testosterone treatment for gender identity disorder (2018)
  • Author(s): Kato K, Abe H, Hanawa N, Fukuzawa J, Matsuo R, Yonezawa T, Itoh S, Sato Y, Ika M, Shimizu S, Endo S, Hano H, Izu A, Sugitani M, Tsubota A
  • Journal Title: Clin J Gastroenterol Volume: Mar 27 Issue: 5 Pages: 401-410
  • DOI: 10.1007/s12328-018-0854-4
  • Peer Reviewed / Open Access
• [Journal Article] Development of a New Conditionally Immortalized Human Liver Sinusoidal Endothelial Cells (2018)
  • Author(s): Zhu M, Koibuchi A, Ide H, Morio H, Shibuya M, Kamiichi A, Tsubota A, Anzai N, Akita H, Chiba K, Furihata T.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 3 Pages: 440-444
  • DOI: 10.1248/bpb.b17-00661
  • NAID: 130006407348
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Efficacy and safety of ombitasvir/paritaprevir/ritonavir combination therapy for genotype 1b chronic hepatitis C patients complicated with chronic kidney disease (2018)
  • Author(s): Arai T, Atsukawa M, Tsubota A, Ikegami T, Shimada N, Kato K, Abe H, Okubo T, Itokawa N, Kondo C, Mikami S, Asano T, Chuganji Y, Matsuzaki Y, Toyoda H, Kumada T, Iio E, Tanaka Y, Iwakiri K.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 7 Pages: 549-555
  • DOI: 10.1111/hepr.13058
  • Peer Reviewed
• [Journal Article] Serum Wisteria floribunda agglutinin-positive Mac-2 binding protein more reliably distinguishes liver fibrosis stages in non-alcoholic fatty liver disease than serum Mac-2 binding protein (2018)
  • Author(s): Atsukawa Masanori、Tsubota Akihito、Okubo Tomomi、Arai Taeang、Nakagawa Ai、Itokawa Norio、Kondo Chisa、Kato Keizo、Hatori Tsutomu、Hano Hiroshi、Oikawa Tsunekazu、Emoto Naoya、Abe Masanori、Kage Masayoshi、Iwakiri Katsuhiko
  • Journal Title: Hepatol Res Volume: in press Issue: 6 Pages: 424-432
  • DOI: 10.1111/hepr.13046
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis of factors predicting the response to tolvaptan in patients with liver cirrhosis and hepatic edema (2017)
  • Author(s): Atsukawa M, Tsubota A, Kato K, Abe H, Shimada N, Asano T, Ikegami T, Koeda M, Okubo T, Arai T, Nakagawa-Iwashita A, Yoshida Y, Hayama K, Itokawa N, Kondo C, Chuganji Y, Matsuzaki Y, Iwakiri K.
  • Journal Title: J Gastroenterol Hepatol Volume: 印刷中 Issue: 6 Pages: 1256-1263
  • DOI: 10.1111/jgh.14047
  • Peer Reviewed
• [Journal Article] Disorders in Hepatic Copper Secretion: Wilson's Disease and Pleomorphic Syndromes (2017)
  • Author(s): Lalioti V, Tsubota A, Sandoval IV.
  • Journal Title: Semin Liver Dis Volume: 37 Issue: 02 Pages: 175-188
  • DOI: 10.1055/s-0037-1602764
  • Peer Reviewed
• [Journal Article] Effectiveness and safety of community-based treatment with sofosbuvir plus ribavirin for elderly patients with genotype 2 chronic hepatitis C (2017)
  • Author(s): Atsukawa M, Tsubota A, Kondo C, Shimada N, Abe H, Kato K, Okubo T, Arai T, Itokawa N, Iio E, Tanaka Y, Iwakiri K.
  • Journal Title: Dig Liver Dis Volume: 49 Issue: 9 Pages: 1029-1035
  • DOI: 10.1016/j.dld.2017.04.012
  • Peer Reviewed
• [Journal Article] Efficacy and safety of ombitasvir/paritaprevir/ritonavir in dialysis patients with genotype 1b chronic hepatitis C (2017)
  • Author(s): Atsukawa M, Tsubota A, Koushima Y, Ikegami T, Watanabe K, Shimada N, Sato S, Kato K, Abe H, Okubo T, Arai T, Itokawa N, Kondo C, Mikami S, Asano T, Chuganji Y, Matsuzaki Y, Iwakiri K.
  • Journal Title: Hepatology Research Volume: 印刷中 Issue: 13 Pages: 1429-1437
  • DOI: 10.1111/hepr.12910
  • Peer Reviewed
• [Journal Article] Clinical evaluation of sofosbuvir/ledipasvir in chronic hepatitis C genotype 1 with and without prior daclatasvir/asnaprevir therapy. (2017)
  • Author(s): Iio E, Shimada N, Takaguchi K, Senoh T, Eguchi Y, Atsukawa M, Tsubota A, Abe H, Kato K, Kusakabe A, Miyaki T, Matsuura K, Matsunami K, Shinkai N, Fujiwara K, Nojiri S, Tanaka Y.
  • Journal Title: Hepatol Res. Volume: 印刷中 Issue: 12 Pages: 1308-1316
  • DOI: 10.1111/hepr.12898
  • Peer Reviewed
• [Journal Article] Retreatment with sofosbuvir, ledipasvir, and add-on ribavirin for patients who failed daclatasvir and asunaprevir combination therapy (2017)
  • Author(s): Suda G, Ogawa K, Yamamoto Y, Katagiri M, Furuya K, Kumagai K, Konno J, Kimura M, Kawagishi N, Ohara M, Umemura M, Ito J, Izumi T, Nakai M, Sho T, Natsuizaka M, Morikawa K, Tsubota A, Shimada N, Iio E, Tanaka Y, Sakamoto N; NORTE Study Group.
  • Journal Title: J Gastroenterol Volume: 印刷中 Issue: 10 Pages: 1122-1129
  • DOI: 10.1007/s00535-017-1328-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Daclatasvir and asunaprevir for genotype 1b chronic hepatitis C patients with chronic kidney disease (2017)
  • Author(s): Kondo C, Atsukawa M, Tsubota A, Shimada N, Abe H, Asano T, Yoshizawa K, Okubo T, Chuganji Y, Aizawa Y, Iio E, Tanaka Y, Iwakiri K.
  • Journal Title: Hepatol Res Volume: 47 Issue: 11 Pages: 1165-1173
  • DOI: 10.1111/hepr.12879
  • Peer Reviewed
• [Journal Article] Clinical Significance of Two Real-Time PCR Assays for Chronic Hepatitis C Patients Receiving Protease Inhibitor-Based Therapy (2017)
  • Author(s): Inoue T, Hmwe SS, Shimada N, Kato K, Ide T, Torimura T, Kumada T, Toyoda H, Tsubota A, Takaguchi K, Wakita T, Tanaka Y.
  • Journal Title: PLoS One Volume: 12 Issue: 1 Pages: e0170667-e0170667
  • DOI: 10.1371/journal.pone.0170667
  • Peer Reviewed / Open Access
• [Journal Article] C-Arm Cone Beam Computed Tomography Guidance for Radiofrequency Ablation in Hepatocellular Carcinoma (2017)
  • Author(s): Kato K, Abe H, Ika M, Yonezawa T, Sato Y, Hanawa N, Shimizu S, Endo S, Matsuo R, Tsubota A.
  • Journal Title: Oncology Volume: 92 Issue: 3 Pages: 142-152
  • DOI: 10.1159/000453665
  • Peer Reviewed
• [Journal Article] Human organic anion transporter 2 is an entecavir, but not tenofovir, transporter (2017)
  • Author(s): Furihata T, Morio H, Zhu M, Suzuki Y, Ide H, Tsubota A, Fu Z, Anzai N, Chiba K.
  • Journal Title: Drug Metab Pharmacokinet Volume: 32 Issue: 1 Pages: 116-119
  • DOI: 10.1016/j.dmpk.2016.09.004
  • NAID: 120006776103
  • Peer Reviewed
• [Journal Article] Efficacy of daclatasvir/asunaprevir according to resistance-associated variants in chronic hepatitis C with genotype 1 (2017)
  • Author(s): Iio E, Shimada N, Abe H, Atsukawa M, Yoshizawa K, Takaguchi K, Eguchi Y, Nomura H, Kuramitsu T, Kang JH, Matsui T, Hirashima N, Tsubota A, Kusakabe A, Hasegawa I, Miyaki T, Shinkai N, Fujiwara K, Nojiri S, Tanaka Y.
  • Journal Title: J Gastroenterol Volume: 52 Issue: 1 Pages: 94-103
  • DOI: 10.1007/s00535-016-1225-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Association between vitamin D deficiency and pre-existing resistance-associated hepatitis C virus NS5A variants (2016)
  • Author(s): 1.Okubo T, Atsukawa M, Tsubota A, Shimada N, Abe H, Yoshizawa K, Arai T, Nakagawa A, Itokawa N, Kondo C, Aizawa Y, Iwakiri K.
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 7 Pages: 641-649
  • DOI: 10.1111/hepr.12784
  • Peer Reviewed
• [Journal Article] Hepatitis C virus G1b infection decreases the number of small low-density lipoprotein particles (2016)
  • Author(s): 2.Kinoshita C, Nagano T, Seki N, Tomita Y, Sugita T, Aida Y, Itagaki M, Satoh K, Sutoh S, Abe H, Tsubota A, Aizawa Y.
  • Journal Title: World J Gastroenterol Volume: 22 Issue: 29 Pages: 6716-25
  • DOI: 10.3748/wjg.v22.i29.6716
  • Peer Reviewed
• [Journal Article] Effects of sorafenib combined with low-dose interferon therapy for advanced hepatocellular carcinoma: a pilot study (2016)
  • Author(s): 3.Itokawa N, Atsukawa M, Tsubota A, Okubo T, Arai T, Nakagawa A, Kondo C, Iwakiri K.
  • Journal Title: Int J Clin Oncol Volume: 21 Issue: 4 Pages: 676-83
  • DOI: 10.1007/s10147-015-0942-0
  • Peer Reviewed
• [Journal Article] Single-Nucleotide Resolution Mapping of Hepatitis B Virus Promoters in Infected Human Livers and Hepatocellular Carcinoma (2016)
  • Author(s): 4.Altinel K, Hashimoto K, Wei Y, Neuveut C, Gupta I, Suzuki AM, Dos Santos A, Moreau P, Xia T, Kojima S, Kato S, Takikawa Y, Hidaka I, Shimizu M, Matsuura T, Tsubota A, Ikeda H, Nagoshi S, Suzuki H, Michel ML, Samuel D, Buendia MA, Faivre J, Carninci P.
  • Journal Title: J Virol Volume: 90 Pages: 10811-22
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Usefulness of portal vein pressure for predicting the effects of tolvaptan in cirrhotic patients (2016)
  • Author(s): Nakagawa A, Atsukawa M, Tsubota A, Kondo C, Okubo T, Arai T, Itokawa N, Narahara Y, Iwakiri K.
  • Journal Title: World J Gastroenterol Volume: 22 Issue: 21 Pages: 5104-13
  • DOI: 10.3748/wjg.v22.i21.5104
  • Peer Reviewed
• [Journal Article] Evaluation of factors associated with relapse in telaprevir-based triple therapy for chronic hepatitis C. (2016)
  • Author(s): 1.Kondo C, Atsukawa M, Tsubota A, Shimada N, Abe H, Aizawa Y.
  • Journal Title: J Postgrad Med Volume: 62 Issue: 1 Pages: 20-25
  • DOI: 10.4103/0022-3859.173191
  • Peer Reviewed
• [Journal Article] Effects of sorafenib combined with low-dose interferon therapy for advanced hepatocellular carcinoma: a pilot study. (2016)
  • Author(s): 2.Itokawa N, Atsukawa M, Tsubota A, Okubo T, Arai T, Nakagawa A, Kondo C, Iwakiri K.
  • Journal Title: Int J Clin Oncol Volume: Epub ahead of print
  • Peer Reviewed
• [Journal Article] Effect of native vitamin D3 supplementation on refractory chronic hepatitis C patients in simeprevir with pegylated interferon/ribavirin. (2016)
  • Author(s): 4.Atsukawa M, Tsubota A, Shimada N, Yoshizawa K, Abe H, Asano T, Ohkubo Y, Araki M, Ikegami T, Okubo T, Kondo C, Osada Y, Nakatsuka K, Chuganji Y, Matsuzaki Y, Iwakiri K, Aizawa Y.
  • Journal Title: Hepatol Res Volume: Epub ahead of print Issue: 5 Pages: 450-458
  • DOI: 10.1111/hepr.12575
  • Peer Reviewed
• [Journal Article] Impact of Chronic Hepatitis C Virus Genotype 1b Infection on Triglyceride Concentration in Serum Lipoprotein Fractions. (2015)
  • Author(s): 3.Nagano T, Seki N, Tomita Y, Sugita T, Aida Y, Itagaki M, Sutoh S, Abe H, Tsubota A, Aizawa Y.
  • Journal Title: Int J Mol Sci Volume: 16 Issue: 9 Pages: 20576-20594
  • DOI: 10.3390/ijms160920576
  • Peer Reviewed
• [Journal Article] Influencing factors on serum 25-hydroxyvitamin D3 levels in Japanese chronic hepatitis C patients. (2015)
  • Author(s): Atsukawa M, Tsubota A, Shimada N, Yoshizawa K, Abe H, Asano T, Ohkubo Y, Araki M, Ikegami T, Kondo C, Itokawa N, Nakagawa A, Arai T, Matsushita Y, Nakatsuka K, Furihata T, Chuganji Y, Matsuzaki Y, Aizawa Y, Iwakiri K.
  • Journal Title: BMC Infect Dis Volume: 15 Issue: 1 Pages: 344-344
  • DOI: 10.1186/s12879-015-1020-y
  • Peer Reviewed
• [Journal Article] Differential inhibition features of direct-acting anti-hepatitis C virus agents against human organic anion transporting polypeptide 2B1. (2015)
  • Author(s): Furihata T, Fu Z, Suzuki Y, Matsumoto S, Morio H, Tsubota A, Matsumoto S, Chiba K.
  • Journal Title: Int J Antimicrob Agents Volume: 46 Issue: 4 Pages: 381-388
  • DOI: 10.1016/j.ijantimicag.2015.05.013
  • Peer Reviewed
• [Journal Article] Vitamin D-related gene polymorphisms do not influence the outcome and serum vitamin D level in pegylated interferon/ribavirin therapy combined with protease inhibitor for patients with genotype 1b chronic hepatitis C. (2015)
  • Author(s): Arai T, Atsukawa M, Tsubota A, Kondo C, Shimada N, Abe H, Itokawa N, Nakagawa A, Okubo T, Aizawa Y, Iwakiri K.
  • Journal Title: J Med Virol Volume: 87 Issue: 11 Pages: 1904-1912
  • DOI: 10.1002/jmv.24244
  • Peer Reviewed
• [Journal Article] Interferon-λ3 polymorphisms in pegylated-interferon-α plus ribavirin therapy for genotype-2 chronic hepatitis C. (2015)
  • Author(s): Ishiguro H, Abe H, Seki N, Sugita T, Aida Y, Itagaki M, Sutoh S, Shimada N, Furihata T, Tsubota A, Aizawa Y.
  • Journal Title: World J Gastroenterol Volume: 21 Issue: 13 Pages: 3904-3911
  • DOI: 10.3748/wjg.v21.i13.3904
  • Peer Reviewed
• [Journal Article] Safety and Efficacy of Partial Splenic Embolization in Telaprevir-based Triple Therapy for Chronic Hepatitis C (2015)
  • Author(s): Kondo C, Atsukawa M, Tsubota A, Shimada N, Abe H, Itokawa N, Nakagawa A, Fukuda T, Matsushita Y, Nakatsuka K, Kawamoto C, Iwakiri K, Aizawa Y, Sakamoto C.
  • Journal Title: Internal Medicine Volume: 54 Issue: 2 Pages: 119-126
  • DOI: 10.2169/internalmedicine.54.3066
  • NAID: 130004903010
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Factors associated with sustained virological response in 24-week telaprevir-based triple therapy for chronic hepatitis C genotype 1b patients with the IL28B minor genotype (2015)
  • Author(s): Abe H, Tsubota A, Shimada N, Atsukawa M, Kato K, Takaguchi K, Asano T, Chuganji Y, Sakamoto C, Toyoda H, Kumada T, Ide T, Sata M, Aizawa Y.
  • Journal Title: Hepatol Res Volume: 45 Issue: 4 Pages: 387-36
  • DOI: 10.1111/hepr.12360
  • Peer Reviewed
• [Presentation] Ultrastructure of hepatocytes in chronic hepatitis C patients who achieve a sustained virological response (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Xin Z, Kao YT, Hossam GE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: APASL 2017 (The 26th Conference of Asian Pacific Association for the Study of the Liver)
  • Place of Presentation: Beijing, China
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Ultrastructure of hepatocytes in chronic hepatitis C patients who achieve a sustained virological response (2017)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, Takuma Zaitsu, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Noritomo Shimada, Keizo Kato, Akihito Tsubota, Ayako Mimata, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Takaji Wakita, Hideki Aizaki
  • Organizer: APASL 2017 (26th)
  • Int'l Joint Research
• [Presentation] Abnormal Hepatocellular Organelles Remain to be Observed in Sustained Virological Response Patients (2017)
  • Author(s): Haruyo Aoyagi, Hiroko Iijima, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, Takuma Zaitsu, Mami Matsuda, Koichi Watashi, Ryosuke Suzuki, Takahiro Masaki, Akihito Tsubota, Mimata Ayako, Yuriko Sakamaki, Shizuko Ichinose, Kenjiro Wake, Takaji Wakita, Hideki Aizaki
  • Organizer: APASL Single Topic Conference
  • Int'l Joint Research
• [Presentation] SVR後の肝臓のオルガネラ異常の解析 (2017)
  • Author(s): 青柳東代, 飯島尋子, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, 在津拓馬, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 島田紀朋, 加藤慶三, 坪田昭人, 三又絢子, 酒巻有里子, 市野瀬志津子, 和氣健二郎, 脇田隆字, 相崎英樹.
  • Organizer: 第27回抗ウイルス療法学会総会
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Xin Z, Kao Y-T, Hossam GE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Aizawa N, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] A novel device for estimating olfactory threshold for differential diagnosis of parkinsonian syndrome: a pilot study (2017)
  • Author(s): Omoto S, Mori E, Fujioka K, Tsubota A, Kita J, Kinoshita M, Matsuno H, Sakai K, Toyoda C, Iguchi Y
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化 (2016)
  • Author(s): 青柳東代, 飯島尋子, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 坪田昭人, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第41回日本肝臓学会東部会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2016-12-08
• [Presentation] Efficacy and safety of ombitasvir/ritonavir/paritaprevir combination therapy for genotype 1b chronic hepatitis C patients complicated with chronic kidney disease (2016)
  • Author(s): Arai T, Atsukawa M, Ikegami T, Mikami S, Shimada N, Tsubota A, Kato K, Abe H, Okubo T, Nakagawa A, Itokawa N, Kondo C, Iwakiri K
  • Organizer: AASLD 2016 (The Liver Meeting, the 67th Conference of American Association for the Study of Liver Diseases)
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] C型慢性肝炎における背景因子の特徴による薬剤選択 (2016)
  • Author(s): 厚川正則, 島田紀朋, 大久保知美, 安部 宏, 加藤慶三, 三上 繁, 坪田昭人, 池上 正, 中願寺義通, 岩切勝彦
  • Organizer: 第20回日本肝臓学会大会
  • Place of Presentation: 神戸コンベンションセンター（神戸）
  • Year and Date: 2016-11-04
• [Presentation] 本邦におけるNAFLD患者と血清ビタミンD代謝 (2016)
  • Author(s): 新井泰央, 厚川正則, 大久保知美, 中川 愛, 糸川典夫, 近藤千紗, 加藤慶三, 坪田昭人, 岩切勝彦
  • Organizer: 第20回日本肝臓学会大会
  • Place of Presentation: 神戸コンベンションセンター（神戸）
  • Year and Date: 2016-11-03
• [Presentation] Ultrastructure of liver cells in chronic hepatitis C patients who achieve a sustained virological response (2016)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Xin Z, Kao YT, Hossam GE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: The 15th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan
  • Year and Date: 2016-09-07
  • Int'l Joint Research
• [Presentation] C型肝炎ウイルス治療後ウイルス学的著効症例の肝細胞の超微細構造の観察 (2016)
  • Author(s): 相崎英樹, 飯島尋子, 青柳東代, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 藤井さやか, 會澤信弘, 三又絢子, 酒巻有里子, 市野瀬志津子, 坪田昭人, 和氣健二郎, 脇田隆字
  • Organizer: 第23回肝細胞研究会
  • Place of Presentation: 大阪大学中之島センター（大阪）
  • Year and Date: 2016-07-08
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化 (2016)
  • Author(s): 青柳東代, 飯島尋子, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 酒巻有里子, 市野瀬志津子, 坪田昭人, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第12回広島肝臓プロジェクト研究センターシンポジウム
  • Place of Presentation: ホテルグランヴィア広島（広島）
  • Year and Date: 2016-06-25
• [Presentation] 実臨床におけるGenotype 2のC型慢性肝炎患者に対するソホスブビル, リバビリン併用療法の治療成績 (2016)
  • Author(s): 近藤千紗, 厚川正則, 島田紀朋, 加藤慶三, 安部 宏, 大久保知美, 新井泰央, 中川 愛, 糸川典夫, 坪田昭人, 相澤良夫, 岩切勝彦
  • Organizer: 第52回日本肝臓学会総会
  • Place of Presentation: ホテルニューオータニ幕張/東京ベイ幕張ホール（千葉）
  • Year and Date: 2016-05-20
• [Presentation] Genotype１C型慢性肝疾患に対するダクラタスビル・アスナプレビルの初期治療反応と抗ウイルス効果の関係 (2016)
  • Author(s): 加藤慶三, 安部 宏, 井家麻紀子, 清水晶平, 佐藤祥之, 望月 司, 塙 紀子, 米澤 健, 遠藤慎治, 松尾亮太, 坪田昭人
  • Organizer: 第52回日本肝臓学会総会
  • Place of Presentation: ホテルニューオータニ幕張/東京ベイ幕張ホール（千葉）
  • Year and Date: 2016-05-19
• [Presentation] HCVに対する抗ウイルス治療後、SVR後の肝細胞の超微細構造の変化 (2016)
  • Author(s): 青柳東代, 飯島尋子, 渡士幸一, 鈴木亮介, 政木隆博, 坂巻有里子, 市野瀬志津子, 坪田昭人, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第26回抗ウイルス療法学会総会
  • Place of Presentation: 名古屋市立大学（名古屋）
  • Year and Date: 2016-05-13
• [Presentation] Serum 25(OH)D3 levels are related to the severity of liver fibrosis in patients with NAFLD (2016)
  • Author(s): Arai T, Atsukawa M, Kondo C, Itokawa N, Nakagawa A, Okubo T, Kato K, Tsubota A, Iwakiri K
  • Organizer: APASL (2016 in Tokyo, 25th Conference)
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2016-02-23
  • Int'l Joint Research
• [Presentation] Efficacy of daclatasvir/asunaprevir for chronic hepatitis C patients with renal insufficiency (2016)
  • Author(s): Kondo C, Atsukawa M, Shimada N, Tsubota A, Abe H, Okubo T, Nakagawa A, Arai T, Itokawa N, Tanaka Y, Aizawa Y, Iwakiri K
  • Organizer: APASL (2016 in Tokyo, 25th Conference)
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2016-02-22
  • Int'l Joint Research
• [Presentation] C型慢性肝疾患に対するダクラタスビル･アスナプレビルの初期治療効果の検討 (2015)
  • Author(s): 加藤慶三,塙 紀子,米澤 健,佐藤祥之,井家麻紀子,清水昌平,松尾亮太,坪田昭人
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] DAAsに対するNS5A領域の薬剤耐性変異を有する症例の特徴 (2015)
  • Author(s): 大久保知美,厚川正則,島田紀朋,安部宏,吉澤海,新井康央,中川愛,糸川典夫,近藤千紗,相澤良夫,坪田昭人,岩切勝彦
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] SMV3剤併用療法の難治要因の検討 (2015)
  • Author(s): 島田紀朋,厚川正則,田中靖人,坪田昭人
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] DCV+ASV併用療法の薬剤耐性変異別の治療効果及びAFPの推移 (2015)
  • Author(s): 島田紀朋,厚川正則,安部宏,吉澤海,田中靖人,坪田昭人,相澤良夫
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] 治療開始4週後のHCV RNA量から見たSMV3剤併用療法の治療効果予測 (2015)
  • Author(s): 島田紀朋,厚川正則,安部宏,吉澤海,高口浩一,石川達,辻邦彦,熊田卓,飯尾悦子,田中靖人,平岡淳,坪田昭人,相澤良夫
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] C型慢性肝炎に対するDCV/ASV療法の成績に与える因子の解析 (2015)
  • Author(s): 厚川正則,島田紀朋,吉澤海,安部宏,大久保知美,新井康央,中川愛,糸川典夫,近藤千紗,坪田昭人,相澤良夫,岩切勝彦
  • Organizer: 第19回日本肝臓学会大会
  • Place of Presentation: グランドプリンスホテル新高輪･国際館パミール（東京）
  • Year and Date: 2015-10-08
• [Presentation] DAAsに対するNS5A領域の薬剤耐性変異を有する症例の特徴 (2015)
  • Author(s): 大久保知美,厚川正則,島田紀朋,吉澤海,安部宏,新井康央,中川愛,糸川典夫,近藤千紗,相澤良夫,坪田昭人
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: 鶴屋東館（熊本）
  • Year and Date: 2015-05-22
• [Presentation] C型慢性肝炎に対するDCV/ASV療法の成績にHost因子が与える影響 (2015)
  • Author(s): 厚川正則,島田紀朋,吉澤海,安部宏,大久保知美,新井康央,中川愛,糸川典夫,近藤千紗,松下洋子,坪田昭人,相澤良夫
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: 鶴屋東館（熊本）
  • Year and Date: 2015-05-22
• [Presentation] 肝硬変に伴う腹水に対するトルバプタンの治療成績 (2015)
  • Author(s): 加藤慶三,塙 紀子,米澤 健,佐藤祥之,井家麻紀子,清水昌平,松尾亮太,坪田昭人
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: 鶴屋東館（熊本）
  • Year and Date: 2015-05-22
• [Presentation] SMV３剤併用療法の成績から見た難治要因の検討 (2015)
  • Author(s): 島田紀朋,厚川正則,坪田昭人,吉澤海,安部宏,加藤慶三,大久保雄介,平岡淳,高口浩一,石川達,辻邦彦,豊田秀徳,熊田卓,田中靖人,相澤良夫
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: ホテル日本航空（熊本）
  • Year and Date: 2015-05-21
• [Presentation] 静脈瘤治療に対する抗ウイルス治療の影響 (2015)
  • Author(s): 加藤慶三,島田紀朋,坪田昭人
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2015-04-24
• [Presentation] Genotype 1b C型慢性肝炎に対するSimeprevir+Peginterferon+Ribavirin３剤療法の早期治療効果 (2015)
  • Author(s): 杉田知典,安部 宏,富田陽一,永野智久,関 伸嘉,会田雄太,板垣宗徳,須藤 訓,坪田昭人,相澤良夫
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2015-04-24
• [Presentation] IFN反応性とウイルス変異から見た治療戦略 (2015)
  • Author(s): 島田紀朋,厚川正則,坪田昭人
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2015-04-23
• [Presentation] C型慢性肝炎治療におけるvitamin Dの影響～vitamin D併用SMV３剤併用療法RCTから得られた知見～ (2015)
  • Author(s): 厚川正則,島田紀朋,坪田昭人
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 仙台国際センター（仙台）
  • Year and Date: 2015-04-23