| Project/Area Number |
15K06816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Mochida Keiji 国立研究開発法人理化学研究所, バイオリソースセンター, 専任技師 (60312287)
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| Research Collaborator |
Hasegawa Ayumi 国立研究開発法人理化学研究所, バイオリソースセンター, テクニカルスタッフⅡ
Kito Seiji 国立大学法人名古屋大学, 動物実験支援センター・東山動物実験施設, 施設長
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 凍結保存 / 冷蔵保存 / 胚 / 精子 / マウス / 輸送 / 受精卵 / マウス輸送 / 寒剤 / 氷晶形成抑制 |
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| Outline of Final Research Achievements |
We established the conditions of simple preservation method for mouse embryos and spermatozoa without liquid nitrogen and dry ice at -40℃ and -2℃. Then, we established the conditions and methods of preservation at temperature ranges from -5℃ to 37℃. Especially, we succeeded to obtaining healthy offspring from both embryos which were stored in refrigerator for 7 days or transported using a commercial service at refrigerated temperature within 5 days. By these technical development, the expense of the transportation for mouse embryos from Japan to Europe or United States of America can reduce to 1/10 - 1/20. Additionally, we succeeded in choosing two candidate genes about cryopreservation sensitivity of the sperm as a more progressive study. Finally, we could establish several new technologies for practical use and mechanism analysis of the cryopreservation and the refrigeration transportation by this funded project.
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