Development of simple preservation and transportation method for mouse embryos and spermatozoa without liquid nitrogen and dry ice
| Project/Area Number |
15K06816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Mochida Keiji 国立研究開発法人理化学研究所, バイオリソースセンター, 専任技師 (60312287)
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| Research Collaborator |
Hasegawa Ayumi 国立研究開発法人理化学研究所, バイオリソースセンター, テクニカルスタッフⅡ
Kito Seiji 国立大学法人名古屋大学, 動物実験支援センター・東山動物実験施設, 施設長
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 凍結保存 / 冷蔵保存 / 胚 / 精子 / マウス / 輸送 / 受精卵 / マウス輸送 / 寒剤 / 氷晶形成抑制 |
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| Outline of Final Research Achievements |
We established the conditions of simple preservation method for mouse embryos and spermatozoa without liquid nitrogen and dry ice at -40℃ and -2℃. Then, we established the conditions and methods of preservation at temperature ranges from -5℃ to 37℃. Especially, we succeeded to obtaining healthy offspring from both embryos which were stored in refrigerator for 7 days or transported using a commercial service at refrigerated temperature within 5 days. By these technical development, the expense of the transportation for mouse embryos from Japan to Europe or United States of America can reduce to 1/10 - 1/20. Additionally, we succeeded in choosing two candidate genes about cryopreservation sensitivity of the sperm as a more progressive study. Finally, we could establish several new technologies for practical use and mechanism analysis of the cryopreservation and the refrigeration transportation by this funded project.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene2017
Author(s)
Michiko Hirose, Ayumi Hasegawa, Keiji Mochida, Shogo Matoba, Yuki Hatanaka, Kimiko Inoue, Tatsuhiko Goto, Hideki Kaneda, Ikuko Yamada, Tamio Furuse, Kuniya Abe, Yoshihisa Uenoyama, Hiroko Tsukamura, Shigeharu Wakana, Arata Honda, and Atsuo Ogura
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42476-42476
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 抗インヒビン血清による過剰排卵と凍結精子用の体外受精法を用いた効率的な受精卵の獲得2016
Author(s)
持田慶司, 長谷川歩未, 大高直樹, 市川英里, 田熊究一, 田中めぐみ, 山村竜典, 竹内隆二, 平岩典子, 吉木淳, 渡辺元, 小倉淳郎
Organizer
第63回日本実験動物学会総会
Place of Presentation
ミューザ川崎シンフォニーホール（神奈川県川崎市）
Year and Date
2016-05-19
Related Report
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