• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of functional genes in outer hair cells using an inducible depletion mouse model

Research Project

Project/Area Number 15K06818
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

MATSUOKA Kunie  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40291158)

Co-Investigator(Renkei-kenkyūsha) KIKKAWA Yoshiaki  公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, プロジェクトリーダー (20280787)
SHITARA Hiroshi  公益財団法人東京都医学総合研究所, 基盤技術研究センター, 主席基盤技術研究職員 (90321885)
WADA Kenta  東京農業大学, 生物産業学部, 准教授 (20508113)
Research Collaborator MIYASAKA Yuki  名古屋大学, 医学系研究科, 助教 (30778098)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords難聴 / 疾患モデル / 内耳外有毛細胞 / RNA-seq / CRISPR/Cas9システム / 疾患モデルマウス
Outline of Final Research Achievements

In cochlea, outer hair cells (OHCs) play an essential role in the amplification of sound-induced vibrations. We generated a mouse model that lacks OHCs by administration of diphtheria toxin. We performed differential expression analysis of cochlea-derived mRNAs isolated from the OHC-depleted and control mice using by RNA-seq and microarray methods. We found that the expression of several genes was remarkably decreased in cochlea of OHC-depleted mice. The downregulated genes included causative genes for hearing impairment, such as Myo15, Strc, Pou4f3, Kcnq4, and Slc26a5. Moreover, we identified that the expression of Ocm, Chrna10, Ppp1r17, and Slc6a11, which was also significantly decreased in cochlea of the OHC-depleted mice. Immunohistochemical analysis showed that those genes were exactly expressed in OHCs. Thus, our study provided expression data of several genes that may have potential functional roles in OHCs.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 2016 2015 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] A novel splice site mutation of myosin VI in mice leads to stereociliary fusion caused by disruption of actin networks in the apical region of inner ear hair cells2017

    • Author(s)
      Seki Yuta、Miyasaka Yuki、Suzuki Sari、Wada Kenta、Yasuda Shumpei P.、Matsuoka Kunie、Ohshiba Yasuhiro、Endo Kentaro、Ishii Rie、Shitara Hiroshi、Kitajiri Shin-ichiro、Nakagata Naomi、Takebayashi Hirohide、Kikkawa Yoshiaki
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 8 Pages: e0183477-e0183477

    • DOI

      10.1371/journal.pone.0183477

    • NAID

      120007099799

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 誘導型破壊マウスを用いた内耳外有毛細胞における機能遺伝子の同定2018

    • Author(s)
      松岡邦枝,安田俊平,和田健太,宮坂勇輝,設楽浩志,多屋長治,吉川欣亮
    • Organizer
      第65回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カドヘリン23のc.753遺伝子型が異なるC57BL/6Jマウス間の加齢に伴う聴力表現型の比較2017

    • Author(s)
      鈴木沙理,宮坂勇輝,関優太,安田俊平,松岡邦枝,設楽浩志,吉川欣亮
    • Organizer
      第64回日本実験動物学会総会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Ca2+結合タンパク質Oncomodulinは内耳外有毛細胞の維持に機能する2017

    • Author(s)
      松岡邦枝,宮坂勇輝,和田健太,安田俊平,設楽浩志,多屋長治,吉川欣亮
    • Organizer
      第64回日本実験動物学会総会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 遺伝子改変マウスを用いて聴覚細胞で機能する遺伝子を探る2017

    • Author(s)
      吉川欣亮,安田俊平,設楽浩志,松岡邦枝
    • Organizer
      第64回日本実験動物学会総会
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Research-status Report
  • [Presentation] カドヘリン 23 の c.753 遺伝子型が異なる C57BL/6J マウス間の加齢に伴う聴力表現型の比較2017

    • Author(s)
      鈴木沙理,宮坂勇輝,関優太,安田俊平,松岡邦枝,設楽浩志,吉川欣亮
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Ca2+ 結合タンパク質 Oncomodulin は内耳外有毛細胞の維持に機能する2017

    • Author(s)
      松岡邦枝,宮坂勇輝,和田健太,安田俊平,設楽浩志,多屋長治,吉川欣亮
    • Organizer
      第64回日本実験動物学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 外有毛細胞特異的遺伝子Oncomodulinノックアウトマウスにおける聴覚障害2016

    • Author(s)
      松岡邦枝,宮坂勇輝,和田健太,安田俊平,設楽浩志,山口碧,多屋長治,吉川欣亮
    • Organizer
      第63回日本実験動物学会総会
    • Place of Presentation
      ミューザ川崎シンフォニーホール(神奈川県川崎市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report 2015 Research-status Report
  • [Presentation] 内耳外有毛細胞選択的破壊マウスを用いた特異遺伝子の探索2015

    • Author(s)
      松岡邦枝,安田俊平,宮坂勇輝,和田健太,設楽浩志,山口碧,多屋長治,吉川欣亮
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス成熟内耳におけるLrrc30の高発現と加齢性難聴抑制効果の関連を探る!2015

    • Author(s)
      宮坂勇輝,安田俊平,関優太,松岡邦枝,日比野浩,木南凌,吉川欣亮
    • Organizer
      第62回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Remarks] 公益財団法人東京都医学総合研究所 哺乳類遺伝プロジェクト

    • URL

      http://www.igakuken.or.jp/mammal/

    • Related Report
      2017 Annual Research Report 2015 Research-status Report
  • [Remarks] 公益財団法人東京都医学総合研究所 哺乳類遺伝プロジェクト

    • URL

      http://www.igakuken.or.jp/mammal/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi