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Profiling of human ovarian cancer using comprehensive microRNA analysis and clarify of mechanism.

Research Project

Project/Area Number 15K06820
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor biology
Research InstitutionHirosaki University

Principal Investigator

Horie Kayo  弘前大学, 保健学研究科, 助教 (30626825)

Co-Investigator(Kenkyū-buntansha) 渡邉 純  弘前大学, 保健学研究科, 教授 (10201188)
Research Collaborator Yokoyama Yoshihito  
Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords卵巣癌 / エクソソーム / miRNA / microRNa / microRNA / micro array
Outline of Final Research Achievements

Exosomes are small vesicles secreted by most cells. Endogenous microRNAs (miRNAs) play critical roles in many biological processes, including tumor growth, apoptosis and tumor genesis . However, the function of endogenous miRNAs of ovarian cancer exosomes is not entirely clear.  This study aimed to clarify the profile of exosomal endogenous miRNA in ovarian cancer cell lines. We established the analysis method of exosomal endogenous miRNA extracted from culture media of ovarian cancer cell lines and profiles were developed for the miRNAs of exosomes in ovarian cancer from the cell lines of various histological cell types. Finally, we narrowed down to three types of candidate miRNAs whose expression was specifically increased in ovarian cancer cell lines.

Academic Significance and Societal Importance of the Research Achievements

本研究では卵巣癌細胞から放出されるエクソソーム内在miRNAをターゲットとしており、新規の卵巣癌の腫瘍増殖・増殖抑制、浸潤に関わる因子を検出できる可能性が高い。そこで、申請者は卵巣癌におけるエクソソーム内在miRNA発現プロファイルを検出することが必要と考え、培養細胞の培養上清から候補因子となるエクソソーム内在miRNAを検出した。その中からマイクロアレイ解析で再現性が認められる候補miRNAを3種に絞り込んだ。またデータベース(Target scan) を用い、候補 miRNAのターゲットとなるmRNAの予測を行い総合的な解析を行うことで腹膜播種の作用機序解明に繋げていくことを考えている。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2018 2017 2016 2015

All Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Presentation] An Investigation of Exosomal Endogenous MicroRNA Profiles in Ovarian Cancer.2018

    • Author(s)
      Kayo Horie
    • Organizer
      The 25th Thai-Japanese Workshop in Diagnostic Cytopathology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Profiling of human ovarian cancer using comprehensive microRNA analysis.2017

    • Author(s)
      Kayo Horie
    • Organizer
      International congress of cytology (The 19th)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 卵巣癌バイオマーカーとしてのエクソソーム内在microRNAの検討2017

    • Author(s)
      堀江香代
    • Organizer
      日本臨床細胞学会秋期大会(第56回)
    • Related Report
      2017 Research-status Report
  • [Presentation] Profiling of human ovarian cancer using comprehensive microRNA analysis.2016

    • Author(s)
      堀江香代
    • Organizer
      The 19th International Congress of Cytology
    • Place of Presentation
      パシフィコ横浜 神奈川県 横浜市
    • Year and Date
      2016-05-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト卵巣癌エクソソーム内在miRNAと細胞内miRNA発現プロファイルの探索2016

    • Author(s)
      堀江香代
    • Organizer
      第57回日本臨床細胞学会総会
    • Place of Presentation
      パシフィコ横浜 神奈川県 横浜市
    • Year and Date
      2016-05-29
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒト卵巣癌エクソソーム内在miRNA発現プロファイルの探索2016

    • Author(s)
      堀江香代
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 卵巣癌エクソソーム内在microRNAの探索2015

    • Author(s)
      堀江香代
    • Organizer
      平成27年度 がん若手ワークショップ
    • Place of Presentation
      蓼科グランドホテル 長野県 茅野市
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2020-03-30  

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