Investigation for the superiority of collective cell movement upon the distant metastasis

• Project/Area Number: 15K06822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Akita University
• Principal Investigator: Kuriyama Sei 秋田大学, 医学(系)研究科(研究院), 准教授 (30398226)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 浸潤・転移 / 臓器親和性 / 遠隔転移 / 骨肉腫 / 転移 / 浸潤 / 移動 / 細胞接着 / 集団移動

Outline of Final Research Achievements

Loss of cell-cell adhesion from epithelial cancer often promotes infiltration and metastasis. In our previous report, the modified lung cancer cells, which has stable cell-cell adhesions, actually promote the dissemination to the opposite lungs. In this study, we planned to clarify how the changes of cell-cell adhesion properties affected on the ability of the distant metastasis. The sub-cell lines experimentally induced metastatic characteristics to liver and kidney changes various cell adhesion molecules. Also gene profiling of these sub-cell lines and the parental cells revealed that PEDF gene was upregulated, which was formerly expected as the anti-cancer drug. We made the parental cell line overexpressing PEDF, and tested gene expression, cell migration, and in vivo metastasis. The cells injected into knee joint produced the primary lesions orthotopically, and metastasized to liver and kidney too. We further researched the unexpected mechanism about PEDF-induced metastasis.

Research Products

• [Journal Article] Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and fascilitates chromosome alignment and bi-orientation establishment (2018)
  • Author(s): Itoh G, Ikeda M, Iemura K, Amin MA, Kuriyama S, Tanaka M, Mizuno N, Osakada H, Haraguchi T, Tanaka K
  • Journal Title: Sci Rep. Volume: 8 Issue: 1 Pages: 7003-7003
  • DOI: 10.1038/s41598-018-25175-4
  • Peer Reviewed / Open Access
• [Journal Article] PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetero-oligomerization through interaction with Bid in human colon cancer (2018)
  • Author(s): Kuriyama S, Tsuji T, Sakuma T, Yamamoto T, Tanaka M
  • Journal Title: Cell Death Discov. Volume: 4 Pages: 11-11
  • Peer Reviewed / Open Access
• [Journal Article] The low expression of miR-451 predicts a worse prognosis in non-small cell lung cancer cases. (2017)
  • Author(s): Goto A, Tanaka M, Yoshida M, Umakoshi M, Nanjo H, Shiraishi K, Saito M, Kohno T, Kuriyama S, Konno H, Imai K, Saito H, Minamiya Y, Maeda D.
  • Journal Title: PLoS One. Volume: 12 Issue: 7 Pages: 1-6
  • DOI: 10.1371/journal.pone.0181270
  • NAID: 120007089063
  • Peer Reviewed / Open Access
• [Journal Article] Mesothelial cells create a novel tissue niche that facilitates gastric cancer invasion (2017)
  • Author(s): Tanaka M., Kuriyama S., Itoh G., Maeda D., Goto A., Tamiya Y., Yanagihara K., Yashiro M., Aiba N.
  • Journal Title: Cancer Research Volume: ７７ Issue: 3 Pages: 684-695
  • DOI: 10.1158/0008-5472.can-16-0964
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis (2017)
  • Author(s): Kuriyama S, Tamiya Y, Tanaka M
  • Journal Title: Histochem Cell Biol Volume: １４７ Issue: 1 Pages: 17-26
  • DOI: 10.1007/s00418-016-1486-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of anti-cancer chemical compounds using Xenopus embryos (2016)
  • Author(s): Tanaka M, Kuriyama S, Itoh G, Kohyama A, Iwabuchi Y, Shibata H, Yashiro M, Aiba N
  • Journal Title: Cancer Sci Volume: 107(6) Issue: 6 Pages: 803-11
  • DOI: 10.1111/cas.12940
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] PLEKHN1 promotes apoptosis by enhancing Bax/Bak hetero-oligomerization through the interaction with Bid in human colon cancer (2018)
  • Author(s): Sei Kuriyama
  • Organizer: The joint annual meeting of 70th JSCB and 51st JSDB co-sponsored by APDBN
• [Presentation] PLEKHN1はBidを介したBaxのオリゴマー化によって細胞死を促進する (2017)
  • Author(s): 栗山 正
  • Organizer: 第76回 日本癌学会学術総会
• [Presentation] Collective durotaxis of cranial neural crest cells in Xenopus (2017)
  • Author(s): Sei Kuriyama
  • Organizer: The 50th annual meeting of JSDB
• [Presentation] PLEKHN1 is required for Bax oligomerization in cancer cell line (2016)
  • Author(s): 栗山 正
  • Organizer: 第75回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06