Analysis of novel pathogenesis of cancer-associated fibroblast involved in age-related stromal exosome changes
| Project/Area Number |
15K06841
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
Umezu Tomohiro 東京医科大学, 医学部, 講師(特任) (40385547)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | miRNA / 細胞外小胞 / 骨髄間質細胞 / がん微小環境 / aging / エクソソーム / 加齢性変化 / 多発性骨髄腫 / 骨髄内微小環境 / 加齢 |
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| Outline of Final Research Achievements |
Bone marrow stromal cells (BMSCs) and their exosomes are a promising area of cancer therapy; however, little is known about the age of BMSCs from which the exosomes came. Here, we investigated therapeutic effects of BMSC exosomes derived from young and elderly donors.
The exosomes were isolated from conditioned medium of BMSCs. We performed exosomal miRNA profiling, and found that the donor’s age decides senescent changes in BMSC, and exosomes derived from young BMSCs could block MM cell-induced angiogenesis in Matrigel plug. We also noted young BMSC-specific exosomal miRNA, such as miR-340. Of note is that the anti-angiogenic effect was potentiated by modifying exosomes of elderly BMSCs; exosomes are directly transfected with miR-340. Our results suggest that the BMSC exosomes is able to transfer the miRNAs, which have the ability to inhibit angiogenesis. The present study provides a new insight toward exosome-based cancer therapy by reconstruction of BMSC-derived exosomes.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Hidden FLT3-D835Y clone in FLT3-ITD-positive acute myeloid leukemia that evolved into very late relapse with T-lymphoblastic leukemia2017
Author(s)
Katagiri S, Umezu T, Asano M, Akahane D, Azuma K, Makishima H, Yoshida K, Watatani Y, Chiba K, Miyano S, Ogawa S, Ohyashiki JH, Ohyashiki K
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Journal Title
Leukemia and Lymphoma
Volume: 3
Issue: 6
Pages: 1-4
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Treatment-free molecular remission achieved by combination therapy with imatinib and IFNα in CML with <i>BIM</i> deletion polymorphism relapsed after stop imatinib2015
Author(s)
Katagiri S, Tauchi T, Umezu T, Saito Y, Suguro T, Asano M, Yoshizawa S, Kitahara T, Akahane D, Tanaka Y, Fujimoto H, Okabe S, Gotoh M, Ito Y, Ohyashiki JH, Ohyashiki K
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Journal Title
Rinsho Ketsueki
Volume: 56
Issue: 2
Pages: 216-219
DOI
NAID
ISSN
0485-1439, 1882-0824
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[Presentation] Exosomal miRNA signature of bone marrow mesenchymal stromal cells derived from MDS patients.2015
Author(s)
Saitoh Y, Imanishi S, Umezu T, Yoshizawa S, Asano M, Fujimoto H, Akahane D, Yamamoto Y, Kobayashi C, Ohyashiki JH, Ohyashiki K
Organizer
第77回日本血液学会学術集会学術集会
Place of Presentation
金沢
Year and Date
2015-10-16
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