Elucidation of the mechanism for regulating microenvironment and cancer development by mutant KRAS
| Project/Area Number |
15K06847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOTAKE Yojiro 近畿大学, 産業理工学部, 准教授 (90531963)
SHIRASAWA Senji 福岡大学, 医学部, 教授 (10253535)
SASAKI Takamitsu 福岡大学, 医学部, 講師 (00382284)
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| Research Collaborator |
HIROSE Yumiko
ISOYAMA Chiyo
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | KRAS / ３次元培養 / 低分子化合物 / 3次元培養 / 化合物スクリーニング |
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| Outline of Final Research Achievements |
In this study, we tried to find the compounds through the elucidation of the mechanism for regulating microenvironment and cancer development by the mutated KRAS. Three-dimensional (3D) floating culture system using the isogenic cell lines expressing wild-type KRAS or mutant (mt) KRAS were established from HKe3 cells, derived from colorectal cancer (CRC) HCT116 cells and disrupted at mtKRAS gene, and employed for the drug screening. During screening for 400 core-compounds from natural products which selectively suppress growth of human CRC spheroids with mtKRAS, we found 121 derivatives from 8 core-compounds and obtain a patent as inhibitors for signalling pathway associated with mtKRAS.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] An Alpha-kinase 2 Gene Variant Disrupts Filamentous Actin Localization in the Surface Cells of Colorectal Cancer Spheroids2017
Author(s)
12.Nishi K, Luo H, Nakabayashi K, Doi K, Ishikura S, Iwaihara Y, Yoshida Y, Tanisawa K, Arai T, Mori S, Sawabe M, Muramatsu M, Tanaka M, Sakata T, Shirasawa S, Tsunoda T
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Journal Title
Anticancer Research
Volume: 37
Issue: 7
Pages: 3855-3862
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells.2016
Author(s)
Ishikura S, Tsunoda T, Nakabayashi K, Doi K, Koyanagi M, Hayashi K, Kawai T, Tanaka Y, Iwaihara Y, Luo H, Nishi K, Okamura T, Shirasawa S.
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Journal Title
Biochim Biophys Acta.
Volume: 1859(11)
Issue: 11
Pages: 1398-1410
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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