Development of a method to identify single molecule adducts generated on genomic DNA
| Project/Area Number |
15K06849
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Toyo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOTSUKA Yukari 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (40373401)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA付加体 / 変異原物質 / 次世代シークエンサー / 第三世代シークエンサー / 一分子検出 / 第三世代シーケンサー |
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| Outline of Final Research Achievements |
It is well documented that DNA adducts are associated with carcinogenesis. The 3rd generation of sequencing technology will enable us to determine formation of adducts on DNA molecule. We synthesized an oligonucleotide harboring one base of DNA-adduct, and performed sequencing analysis. Polymerase elongation delay was observed at the site of DNA adducts. Next, we enriched DNA fragments including adducts by using immuno-precipitation method because the amount of DNA adducts occurring in the genomic DNA was limited. By combining these methods, the relationship between adduct formation and mutations at critical positions would be clarified.
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Report
(4 results)
Research Products
(10 results)
