| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It is well documented that DNA adducts are associated with carcinogenesis. The 3rd generation of sequencing technology will enable us to determine formation of adducts on DNA molecule. We synthesized an oligonucleotide harboring one base of DNA-adduct, and performed sequencing analysis. Polymerase elongation delay was observed at the site of DNA adducts. Next, we enriched DNA fragments including adducts by using immuno-precipitation method because the amount of DNA adducts occurring in the genomic DNA was limited. By combining these methods, the relationship between adduct formation and mutations at critical positions would be clarified.
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