Development of highly sensitive diagnostic method of gastric cancer using Liquid biopsy and Digital PCR

• Project/Area Number: 15K06857
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: The University of Tokushima (2016-2017); Sapporo Medical University (2015)
• Principal Investigator: SATO Yasushi 徳島大学, 大学院医歯薬学研究部(医学系), 特任教授 (80343383)
• Co-Investigator(Kenkyū-buntansha): 瀧本 理修 札幌医科大学, 医学部, 准教授 (10336399) ; 加藤 淳二 札幌医科大学, 医学部, 教授 (20244345) ; 植村 尚貴 札幌医科大学, 医学部, 研究員 (50749588)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 胃癌 / 診断 / biomarker / Liquid biopsy / Liquid Biopsy

Outline of Final Research Achievements

Currently, there is a desperate need for developing more sensitive, real time detecting method to diagnose gastric cancer in early stage. Because most of the patients diagnosed with gastric cancer are unsuitable for curative surgery since many patients already have distant metastasis at diagnosis, which resulted in extremely poor prognoses. Until now, we reported ST6GalNAc-I that is known as the sialyl-Tn antigen synthase is highly expressed in gastric cancer and related with its malignant potential. We then examined expression of ST6GalNAc-I mRNA in peripheral blood by using digital PCR, so-called "liquid biopsy", and then we verified that the relationship between its copy number expression and their clinical courses. We are now developing a new monitoring biomarker system using this technique for gastric cancer treatment.

Research Products

• [Journal Article] Docetaxel, cisplatin and S-1 (DCS) combination chemotherapy for gastric cancer patients with peritoneal metastasis: a retrospective study (2018)
  • Author(s): Ohnuma Hiroyuki、Sato Yasushi、Hirakawa Masahiro、Kikuchi Shohei、Miyanishi Koji、Sagawa Tamotsu、Takahashi Yasuo、Nobuoka Takayuki、Okamoto Koichi、Miyamoto Hiroshi、Takemasa Ichiro、Takayama Tetsuji、Kato Junji
  • Journal Title: Cancer Chemother Pharmacol. Volume: 81 Issue: 3 Pages: 539-548
  • DOI: 10.1007/s00280-018-3523-x
  • Peer Reviewed
• [Journal Article] A phase II study of modified docetaxel, cisplatin, and S-1 (mDCS) chemotherapy for unresectable advanced gastric cancer (2017)
  • Author(s): Uemura Naoki、Kikuchi Shohei、Sato Yasushi、Ohnuma Hiroyuki、Okamoto Koichi、Miyamoto Hiroshi、Hirakawa Masahiro、Sagawa Tamotsu、Fujikawa Koshi、Takahashi Yasuo、Okuda Toshinori、Minami Shinya、Takahashi Minoru、Okamoto Tetsuro、Takada Kohichi、Miyanisi Koji、Takayama Tetsuji、Kato Junji
  • Journal Title: Cancer Chemother Pharmacol Volume: 80 Issue: 4 Pages: 707-713
  • DOI: 10.1007/s00280-017-3404-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Conversion therapy for inoperable advanced gastric cancer patients by docetaxel, cisplatin, and S-1 (DCS) chemotherapy: a multi-institutional retrospective study (2017)
  • Author(s): Sato Yasushi、Ohnuma Hiroyuki、Nobuoka Takayuki、Hirakawa Masahiro、Sagawa Tamotsu、Fujikawa Koshi、Takahashi Yasuo、Katsuki Shinich、Takahashi Minoru、Maeda Masahiro、Okagawa Yutaka、Naoki Uemura、Kikuch Syouhei、Okamoto Koichi、Miyamoto Hiroshi、Shimada Mitsuo、Ichiro Takemasa、Kato Junji、Takayama Tetsuji
  • Journal Title: Gastric Cancer Volume: 20 Issue: 3 Pages: 517-526
  • DOI: 10.1007/s10120-016-0633-1
  • NAID: 40021185775
  • Peer Reviewed
• [Presentation] A dose-escalation study of docetaxel, oxaliplatin and S-1 (DOS) as a first-line therapy for patients with unresectable metastatic gastric cancer., (2018)
  • Author(s): Yasushi Sato, Tamotsu Sagawa, Yasuo Takahashi, Hiroyuki Ohnuma, Kyoko Hamaguchi, Masahiro Hirakawa, Koshi Fujikawa, Koichi Okamoto, Hiroshi Miyamoto, Naoki Muguruma and Tetsuji Takayama
  • Organizer: Gastrointestinal Cancers Symposium
  • Int'l Joint Research