| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Glycomic analysis focused on sulfated O-glycans was performed to identify novel serum carbohydrate tumor markers for clinical diagnosis. In human sera, the majority of serum oligosaccharides are composed of neutral glycans or terminally sialylated acidic glycans. The elimination of these glycans facilitated the identification of 14 sulfated carbohydrate tumor marker candidates from cancer patient sera. The carbohydrate structures of all marker candidates were precisely analyzed using enzymatic digestion, glycan synthesis, 2D mapping and mass spectrometry. Some candidates have the type1 and type2 lactosamine hybrid backbone. Furthermore, the levels of these sulfated candidates in sera from 11 gastric and 9 pancreatic cancer patients and 20 healthy controls were quantified using mass spectrometry. The levels of all candidates were elevated in sera of at least one or more patients.
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