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Basic Analysis for internal radiation therapy using stem cell

Research Project

Project/Area Number 15K06868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionThe University of Tokyo

Principal Investigator

Nomura Sachiyo  東京大学, 医学部附属病院, 准教授 (70301819)

Co-Investigator(Kenkyū-buntansha) 細谷 紀子  東京大学, 大学院医学系研究科(医学部), 講師 (00396748)
Co-Investigator(Renkei-kenkyūsha) MOMOSE Toshimitsu  東京大学, 医学部附属病院, 准教授 (20219992)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords胃癌 / 腹膜播種 / 内照射 / 間葉系幹細胞 / 放射性ヨード / 骨髄由来間葉系幹細胞 / 胃がん
Outline of Final Research Achievements

We established mouse gastric cancer cell lines that can be transplanted into C57BL/6 mice, and found that cancer associated fibroblast is of bone marrow origin. Gastric cancer peritoneal dissemination model was established with these cell lines. NIS was transfected into bone marrow derived mesenchymal stem cells (BMDC) that were already transfected with SV40T large antigen, and NIS expressing BMDC was established.
CRISPR/CAS9 system was confirmed to use for gene knock-down for the gastric cancer cell lines, and the methods for gathering BMDC around the peritoneal disseminated cancer cells were analyzed. And GFP expressing BMDC was used and the way to gather BMDC efficiently around peritoneal disseminated gastric cancer cells were established.

Report

(3 results)
  • 2017 Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2017 2016

All Presentation (2 results)

  • [Presentation] TFF expression in most virulent mouse gastric cancer cell line transplantable to C57BL/6 mouse.2017

    • Author(s)
      Sachiyo Nomura, Masami Yamamoto, Yasuyuki Seto, Masae Tatematsu, Tetsuya Tsukamoto
    • Organizer
      第89回日本胃癌学会総会
    • Place of Presentation
      広島
    • Related Report
      2016 Research-status Report
  • [Presentation] C57BL/6マウス可移植マウス胃がん細胞高悪性度クローンにおけるTFF1の高発現2016

    • Author(s)
      野村幸世、山本昌美、長岡孝治、松下博和、瀬戸泰之、垣見和弘、立松正衛、塚本哲哉
    • Organizer
      第27回日本消化器癌発生学会総会
    • Place of Presentation
      鹿児島
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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