inhibition of cancer progression by regulation of amino acid transporters

• Project/Area Number: 15K06879
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Kyorin University
• Principal Investigator: Kimura Toru 杏林大学, 医学部, 学内講師 (30433725)
• Research Collaborator: ENDOU Hitoshi 杏林大学, 医学部, 名誉教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: トランスポーター / 癌 / アミノ酸

Outline of Final Research Achievements

Amino acids are essential for our body. Some of the amino acids have a role for signaling molecules and are involved in various reaction in our body. Amino acid transporters has an ability to regulate amino acid gradient across membrane. In this study, we examined amino acid transporters related with cancer progression.
We focused on transporters that transport leucine and arginine important for cell growth and NO production. It was suggested that LAT1 is the most important transporter in cancer growth. We also searched and investigated regulators of LAT1 transporter. We found molecular chaperone and one of the protein related with protein degradation pathway for LAT1 interacting proteins.

Research Products

• [Journal Article] Prostate Cancer Cells in Different Androgen Receptor Status Employ Different Leucine Transporters. (2017)
  • Author(s): Otsuki H, Kimura T, Yamaga T, Kosaka T, Suehiro JI, Sakurai H.
  • Journal Title: Prostate. Volume: 77 Issue: 2 Pages: 222-233
  • DOI: 10.1002/pros.23263
  • Peer Reviewed
• [Journal Article] Inhibition of epithelial-mesenchymal transition by cetuximab via the EGFR-GEP100-Arf6-AMAP1 pathway in head and neck cancer. (2017)
  • Author(s): Matsumoto Y, Sakurai H, Kogashiwa Y, Kimura T, Matsumoto Y, Shionome T, Asano M, Saito K, Kohno N.
  • Journal Title: Head Neck. Volume: 39 Issue: 3 Pages: 476-485
  • DOI: 10.1002/hed.24626
  • Peer Reviewed
• [Journal Article] Up-Regulation of LAT1 during Antiandrogen Therapy Contributes to Progression in Prostate Cancer Cells (2015)
  • Author(s): Xu M, Sakamoto S, Matsushima J, Kimura T, Ueda T, Mizokami A, Kanai Y, Ichikawa T.
  • Journal Title: J Urol Volume: 195 Issue: 5 Pages: 1588-1597
  • DOI: 10.1016/j.juro.2015.11.071
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 前立腺癌アンドロゲン受容体高発現細胞株におけるロイシン輸送トランスポータ (2015)
  • Author(s): 大槻英男、木村 徹、山賀 貴、末弘淳一、櫻井裕之
  • Organizer: 第 132 回日本薬理学会関東部会
  • Place of Presentation: 浦安
  • Year and Date: 2015-07-04