Isolation of antibody set for anticancer agents and development of novel cancer model rat for evaluation of them.

• Project/Area Number: 15K06884
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Fujita Health University
• Principal Investigator: Kuosawa Gene 藤田保健衛生大学, 研究支援推進センター, 講師 (10410739)
• Research Collaborator: TSUDA Hiroyuki 名古屋市立大学, 教授 ; OKA Chieko
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん / がん周辺微小環境 / ヒトRas変異 / 抗体評価系 / ヒト抗体 / 免疫 / モデル動物 / 免疫寛容 / アデノシンパスウェイ / 評価系

Outline of Final Research Achievements

This project aim to develop the next generation human monoclonal antibodies, using spontaneous tumorigenesis rat model via mutated Ras over expression. Currently an immune check point are most important concept for development of anti cancer monoclonal antibody agents. In this regards, the immune check point inhibitor were strongly expected to be next generation anti cancer agents. On the other hand, the evaluation system for these agent are still limited such as a Syngenic grafting model. Therefore this project try to solve this matter using the spontaneous tumorigenesis rat model. Thus, we have isolated antibody set for anticancer agents and development of novel cancer model rat for evaluation of them.

Research Products

• [Journal Article] Uptake of 111In-labeled fully human monoclonal antibody TSP-A18 reflects transferrin receptor expression in normal organs and tissues of mice (2017)
  • Author(s): Sugyo A, Tsuji AB, Sudo H, Nomura F, Satoh H, Koizumi M, Kurosawa G, Kurosawa Y, Saga T
  • Journal Title: Oncol Rep Volume: 37 Issue: 3 Pages: 1529-1536
  • DOI: 10.3892/or.2017.5412
  • Peer Reviewed
• [Journal Article] Development of a complete human IgG monoclonal antibody to transferrin receptor 1 targeted for adult T-cell leukemia/lymphoma. (2017)
  • Author(s): Shimosaki S, Nakahata S, Ichikawa T, Kitanaka A, Kameda T, Hidaka T, Kubuki Y, Kurosawa G, Zhang L, Sudo Y, Shimoda K, Morishita K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 485 Issue: 1 Pages: 144-151
  • DOI: 10.1016/j.bbrc.2017.02.039
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A variety of human monoclonal antibodies against epidermal growth factor receptor isolated from a phage antibody library. (2016)
  • Author(s): Kurosawa Gene, Kondo Mariko, Kurosawa Yoshikazu
  • Journal Title: Biochem Biophys Res Commun. Volume: 480 Issue: 1 Pages: 94-100
  • DOI: 10.1016/j.bbrc.2016.10.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Classification of 27 Tumor-Associated Antigens by Histochemical Analysis of 36 Freshly Resected Lung Cancer Tissues. (2016)
  • Author(s): Kurosawa Gene, Sugiura Mototaka, Hattori Yoshinobu, Tsuda Hiroyuki, Kurosawa Yoshikazu
  • Journal Title: Int J Mol Sci. Volume: 17 Issue: 11 Pages: 1862-1862
  • DOI: 10.3390/ijms17111862
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evaluation of Efficacy of Radioimmunotherapy with 90Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models (2015)
  • Author(s): Aya Sugyo; Atsushi B. Tsuji; Hitomi Sudo; Maki Okada; Mitsuru Koizumi; Hirokazu Satoh; Gene Kurosawa; Yoshikazu Kurosawa; Tsuneo Saga
  • Journal Title: PLoS ONE Volume: 10(4)
  • Peer Reviewed / Open Access / Int'l Joint Research