Is sequence-dependent allele-specific hydroxymethylation ubiquitous in the mammalian genome?

• Project/Area Number: 15K06894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Kanazawa University
• Principal Investigator: yamada yoichi 金沢大学, 電子情報学系, 准教授 (30377402)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: ヒドロキシメチル化

Outline of Final Research Achievements

研究成果の概要（英文）：The research representative previously reported sequence-dependentallele-specific methylation. This enabled us to interpret the mechanisms for differences of various disease susceptibility caused by the sequence polymorphisms. On the other hand, genomic hydroxymethylation plays an important role in the demethylation process. Furthermore, it is suggested that genomic hydroxymethylation is also involved in regulation of gene expression. In this study, we examined whether 10 sequence-dependent allele-specific methylated regions in mice are subject to a sequence-dependentallele-specific hydroxymethylation. As a result, it was shown that one of the ten was subject to a sequence-dependent allele-specific hydroxymethylation.