| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Elucidation of molecular mechanisms underlying genome functions requires identification of molecules interacting with genomic regions of interest in vivo. In this study, we developed in vitro enChIP using purified CRISPR complexes to isolate a target genomic region while retaining molecular interactions. Combining in vitro enChIP with next-generation sequencing analysis, we succeeded in identifying genomic regions that interact with a target locus in vivo. In addition, we showed that an interacting genomic region works as an enhancer to upregulate transcription from the target locus. Thus, in vitro enChIP is a useful tool for elucidation of molecular mechanisms underlying genome functions.
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