Development of in vitro enChIP and its applications to analysis of molecular mechanisms underlying genome functions
| Project/Area Number |
15K06895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | in vitro enChIP / enChIP / locus-specific ChIP / ChIP / CRISPR / TAL / chromatin / chromosomal conformation |
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| Outline of Final Research Achievements |
Elucidation of molecular mechanisms underlying genome functions requires identification of molecules interacting with genomic regions of interest in vivo. In this study, we developed in vitro enChIP using purified CRISPR complexes to isolate a target genomic region while retaining molecular interactions. Combining in vitro enChIP with next-generation sequencing analysis, we succeeded in identifying genomic regions that interact with a target locus in vivo. In addition, we showed that an interacting genomic region works as an enhancer to upregulate transcription from the target locus. Thus, in vitro enChIP is a useful tool for elucidation of molecular mechanisms underlying genome functions.
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Report
(4 results)
Research Products
(25 results)
