Deciphering molecular chromatin dynamics at the chromosomal fragile site via genome editing
Project/Area Number |
15K06896
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | Tottori University |
Principal Investigator |
Nakayama Yuji 鳥取大学, 生命機能研究支援センター, 助教 (40432603)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 脆弱X症候群 / 染色体工学 / ゲノム編集 / トリプレットリピート / CGGリピート |
Outline of Final Research Achievements |
Fragile X syndrome is the most common form of familial intellectual disability, and is caused via expansion (destabilization) of CGG repeat present within the responsible gene FMR1 and gene inactivation accompanying it at the maternal propagation. In the present study, in order to elucidate a series of molecular pathological mechanisms, we tried to establish a system that can monitor the instability of repeat with fluorescence using chromosome engineering technology and genome editing and to identify factors related to it, Fluorescence monitoring system using genome editing failed to obtain the result reflecting the expression pattern of FMR1 originally recognized in humans. However, it was shown that chromosomal engineering operation is a very useful methodology for stable handling of chromosome fragile sites mainly composed of CGG repeats and the like.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease2017
Author(s)
Nagata, K. Kumata, K. Nakayama, Y. Satoh, Y. Sugihara, H. Hara, S. Matsushita, M. Kuwamoto, S. Kato, M. Murakami, I. Hayashi, K.
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Journal Title
Viral Immunol
Volume: 30
Issue: 3
Pages: 240-249
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A high number of IgG4-positive cells in gastric cancer tissue is associated with tumor progression and poor prognosis2016
Author(s)
Miyatani, K. Saito, H. Murakami, Y. Watanabe, J. Kuroda, H. Matsunaga, T. Fukumoto, Y. Osaki, T. Nakayama, Y. Umekita, Y. Ikeguchi, M.
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Journal Title
Virchows Arch
Volume: 468
Issue: 5
Pages: 549-557
DOI
Related Report
Peer Reviewed
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[Journal Article] Carvedilol Suppresses Apoptosis and Ion Channel Remodelling of HL-1 Cardiac Myocytes Expressing E334K cMyBPC.2015
Author(s)
Endo R, Bahrudin U, Notsu T, Tanno S, Onohara T, Yamaguchi S, Ikeda N, Surastri B, Nakayama Y, Ninomiya H, Shirayoshi Y, Inagaki Y, Yamamoto K, Yoshida A, Hisatome I.
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Journal Title
Drug Res (Stuttg).
Volume: 66
Issue: 03
Pages: 126-129
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reactivation of persistent Epstein-Barr virus (EBV) causes secretion of thyrotropin receptor antibodies (TRAbs) in EBV-infected B lymphocytes with TRAbs on their surface2015
Author(s)
Nagata, K. Nakayama, Y. Higaki, K. Ochi, M. Kanai, K. Matsushita, M. Kuwamoto, S. Kato, M. Murakami, I. Iwasaki, T. Nanba, E. Kimura, H. Hayashi, K.
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Journal Title
Autoimmunity
Volume: 48
Issue: 5
Pages: 1-8
DOI
Related Report
Peer Reviewed
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[Presentation] Epstein-Barr virus reactivation induces immunoglobulin production through activation-induced cytidine deaminase expression of B cells from Graves’disease patients and controls.2016
Author(s)
Nagata K, Kumata K, Nakayama Y, Satoh Y, Hara S, Matsushita M, Murakami I, Kuwamoto S, Kato M, Sugihara H, Hayashi K.
Organizer
第45回 日本免疫学会学術集会
Place of Presentation
沖縄
Year and Date
2016-12-05
Related Report
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[Presentation] Biphasic production of immunoglobulin from Epstein-Barr virus reactivated PBMCs in Graves’ disease patients and controls2016
Author(s)
Nagata K, Kumata K, Nakayama Y, Satoh Y, Matsushita M, Murakami I, Kuwamoto S, Kato M, Sugihara H, Hayashi K
Organizer
XXXI International Congress of the International Academy of Pathology and 28th Congress of the European Society of Pathology
Place of Presentation
Cologne, Germany
Year and Date
2016-09-25
Related Report
Int'l Joint Research
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[Presentation] Epstein-Barr virus reactivation stimulates the expression of activation-induced cytidine deaminase in PBMCs from Graves’ disease patients and healthy controls2015
Author(s)
Keiko Nagata, Yuji Nakayama, Yukio Satoh, Keisuke Kumata, Michiko Matsushita, Shunsuke Tanio, Ichiro Murakami, Satoshi Kuwamoto, Masako Kato, Hirotsugu Sugihara, Kazuhiko Hayashi
Organizer
27th European Congress of Pathology
Place of Presentation
Belgrade(セルビア)
Year and Date
2015-09-05
Related Report
Int'l Joint Research
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[Presentation] Epstein-Barr virus reactivation stimulates the expression of activation-induced cytidine deaminase in PBMCs from Graves’ disease patients and healthy controls2015
Author(s)
Keiko Nagata, Yukio Satoh, Yuji Nakayama, Keisuke Kumata, Michiko Matsushita, Shunsuke Tanio, Ichiro Murakami, Satoshi Kuwamoto, Masako Kato, Hirotsugu Sugihara, Kazuhiko Hayashi
Organizer
第12回 EBV研究会
Place of Presentation
出雲市 ビックハート
Year and Date
2015-07-20
Related Report
Int'l Joint Research
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