| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we try to identify RNA molecules that regulates DNA topoisomerase IIβ involved in expression of neural genes close to gene desert. Mutation of the lysine in the regulatory domain (CRD) of Topo IIβ that binds to RNA molecules altered the nuclear localization. We showed that gene expression from a model gene region (rrad gene region in rat chromosome 19) adjacent to the gene desert occurs after chromatin structure change by topo IIβ, and the changes are completely suppressed by ICRF-193, specific inhibitor of topo II.
|
