Epidemiological and experimental analyses of arsenic exposure to epigenome of human embryos and child

• Project/Area Number: 15K06912
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Jichi Medical University
• Principal Investigator: Mise Nathan 自治医科大学, 医学部, 講師 (00360644)
• Research Collaborator: Fatmi Zafar Aga Khan大学, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 無機ヒ素暴露 / DNAメチル化 / エピジェネティクス / 胚発生 / 無機ヒ素 / 胎児期曝露 / ヒ素 / 胎盤 / 胚性幹細胞

Outline of Final Research Achievements

Intake of inorganic arsenic causes various adverse effects through alteration of methylation of genomic DNA. We collected food duplicate samples and genomic DNA samples of children and pregnant women from inorganic arsenic contaminated areas of Pakistan to determine the levels of inorganic arsenic intake and effects on DNA methylation. We have found that the methylation of the promoter regions of lung cancer-related genes, HOXB5, HOXB9, POLD4, and MSX1 were lower in higher arsenic exposed group than in lower exposed group. These results indicate that exposure to inorganic arsenic affects to health of children and pregnant women through aberrant DNA methylation due to competition of general methyl-donor.

Research Products

• [Int'l Joint Research] アガカーン大学(パキスタン)
• [Int'l Joint Research] アガ・カーン大学/Department of Community Health Sciences(パキスタン)
• [Int'l Joint Research] アガ・カーン大学(パキスタン)
• [Journal Article] The cardiac glycoside ouabain activates NLRP3 inflammasomes and promotes cardiac inflammation and dysfunction (2017)
  • Author(s): Kobayashi Motoi、Usui-Kawanishi Fumitake、Karasawa Tadayoshi、Kimura Hiroaki、Watanabe Sachiko、Mise Nathan、Kayama Fujio、Kasahara Tadashi、Hasebe Naoyuki、Takahashi Masafumi
  • Journal Title: PLOS ONE Volume: 12 Issue: 5 Pages: 176676-176676
  • DOI: 10.1371/journal.pone.0176676
  • Peer Reviewed / Open Access
• [Journal Article] Lead Exposure Assessment among Pregnant Women, Newborns, and Children: Case Study from Karachi, Pakistan (2017)
  • Author(s): Fatmi Zafar、Sahito Ambreen、Ikegami Akihiko、Mizuno Atsuko、Cui Xiaoyi、Mise Nathan、Takagi Mai、Kobayashi Yayoi、Kayama Fujio
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 14 Issue: 4 Pages: 413-413
  • DOI: 10.3390/ijerph14040413
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regional differences in infant 25-Hydroxyvitamin D: Pilot study of the Japan Environment and Children’s Study (2017)
  • Author(s): Ayabe T、Yamamoto-Hanada K、Mezawa H、Konishi M、Ishitsuka K、Saito M、Fukami M、Michikawa T、Yamazaki S、Senju A、Kusuhara K、Kawamoto T、Sanefuji M、Kato K、Oda M、Mitsubuchi H、Katoh T、Monden Y、Mise N、Kayama F、Saito H、Ohya Y
  • Journal Title: Pediatrics International Volume: 60 Issue: 1 Pages: 30-34
  • DOI: 10.1111/ped.13410
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The cardiac glycoside ouabain activates NLRP3 inflammasomes and promotes cardiac inflammation and dysfunction (2017)
  • Author(s): Motoi Kobayashi, Fumitake Usui, Tadayoshi Karasawa, Hiroaki Kimura, Sachiko Watanabe, Nathan Mise, Fujio Kayama, Tadashi Kasahara, Naoyuki Hasebe, Masafumi Takahashi
  • Journal Title: PLOS One Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Heavy metal exposure, in combination with physical activity and aging, is related with oxidative stress in Japanese women from a rural agricultural community (2016)
  • Author(s): Cui, X. Ohtsu, M. Mise, N. Ikegami, A. Mizuno, A. Sakamoto, T. Ogawa, M. Machida, M. Kayama, F.
  • Journal Title: Springerplus Volume: 5巻 Issue: 1 Pages: 885-885
  • DOI: 10.1186/s40064-016-2430-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] External lead contamination of women's nails by surma in Pakistan: Is the biomarker reliable? (2016)
  • Author(s): Ikegami, A. Takagi, M. Fatmi, Z. Kobayashi, Y. Ohtsu, M. Cui, X. Mise, N. Mizuno, A. Sahito, A. Khoso, A. Kayama, F.
  • Journal Title: Environmental Pollution Volume: 218巻 Pages: 723-727
  • DOI: 10.1016/j.envpol.2016.07.068
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Exposure assessment of lead from food and airborne dusts and biomonitoring in pregnant mothers, their fetus and siblings in Karachi, Pakistan and Shimotsuke, Japan. (2016)
  • Author(s): Kayama F, Fatmi Z, Ikegami A, Mizuno A, Ohtsu M, Mise N, Cui X, Ogawa M, Sakamoto T, Nakagi Y, Yoshida T, Sahito A, Naeem S, Ghias K, Zuberi H, Tariq K, Kobayashi Y, Nohara K.
  • Journal Title: Rev. Environ. Health. Volume: 31(1) Issue: 1 Pages: 33-35
  • DOI: 10.1515/reveh-2015-0046
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Effects of inorganic arsenic intake on development of infant's groos motor development (2017)
  • Author(s): 三瀬名丹
  • Organizer: 日本衛生学会