| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Human pluripotent stem cell-derived cardiomyocytes show promise for clinical application. To reach this potential, however, improved maturity and reduced cellular heterogeneity of in vitro differentiated cardiomyocytes is needed. Here, we addressed transcriptional heterogeneity in induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iPSC-CMs) by single-cell RNA sequencing. We successfully defined differentiation states at the single-cell level and clarified heterogeneities in the gene expression patterns of iPSC-CMs, especially those cultured over long periods. We developed a novel maturation index to perform in silico sorting and score the maturation level of the cells. Additionally, we identified a new cell surface marker of mature iPSC-CMs by which we could enrich well-matured iPSC-CMs from a heterogeneous population of iPSC-CMs. Our single-cell RNA sequencing approach could help clarify variation of in vitro differentiated cells.
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