| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
It was increased an insertion efficiency of VLox and SLox sites, which are used for site specific recombination, on both sides of the target gene (GOI) by optimizing the reaction conditions of a CRISPR/Cas9 system. However, the efficiency varied greatly among cell types. On the other hand, the efficiency of the GOI into the HAC vector was little difference between cell types, and also succeeded for GOI with a total DNA length of 600 kb. It was suceeded in tarnsferring HAC vector carrying GOI to other cells in all cell types tested . By selecting the host cell type, it was possible to complete the process up to cloning of the large GOI into the HAC vector, and transfer of the HAC vector carrying the GOI to the target cell in 2 months.
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