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Development of isolation method for endophytic actinomycetes using plant model and search for microbial resources

Research Project

Project/Area Number 15K06936
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservation of biological resources
Research InstitutionKitasato University

Principal Investigator

Matsumoto Atsuko  北里大学, 感染制御科学府, 准教授 (20300759)

Co-Investigator(Kenkyū-buntansha) 中島 琢自  北里大学, 北里生命科学研究所, 特任准教授 (40526216)
Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Yoko  北里大学, 北里生命科学研究所, 研究員 (80197186)
SHIOMI Kazuro  北里大学, 感染制御科学府, 教授 (40235502)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords放線菌 / 植物内生菌 / 未利用微生物 / 微生物資源 / 分類 / 植物内生放線菌 / 植物培養細胞 / 共培養
Outline of Final Research Achievements

There are many untapped endophytic actiomycetes. In order to isolate and culture them as source of new bioactive compounds, new isolation method using cultured cell of plant was developed. Endophytic actinomycete strain, which requires cultured cell of plant for growth was isolated by this method.
It was showed by taxonomic study that isolated endophytic actinomycetes were three new species belonging the genus Kibdelosporangium, and the 22-membered macrolides, hamuramicin was found in the cultured broth of endophytic actinomycetes Allostreptomyces sp. K12-0794.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Endophytic actinomycetes: promising source of novel bioactive compounds2017

    • Author(s)
      Matsumoto A. and Takahashi Y.
    • Journal Title

      J. Antibiot.

      Volume: 67 Issue: 5 Pages: 1785-1765

    • DOI

      10.1038/ja.2017.20

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three novel species of the genus Kibdelosporangium; Kibdelosporangium kanagawaense sp. nov., Kibdelosporangium rhizosphaerae sp. nov. and Kibdelosporangium rhizovicinum sp. nov.2017

    • Author(s)
      Mingma R., Duangmal K., Omura S. Takahashi Y.and Matsumoto A.
    • Journal Title

      Int. J. Syst. Evol. Microbiol.

      Volume: 67 Issue: 6 Pages: 1758-1765

    • DOI

      10.1099/ijsem.0.001861

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Structural Analyses of Cell-Wall Peptidoglycans of Actinomycetes Belonging To the Family Micromonosporaceae2017

    • Author(s)
      Akira Take, Takuji Nakashima, Yuki Inahashi, Satoshi Omura, Kazuro Shiomi, Yoko Takahashi and Atsuko Matsumoto
    • Organizer
      International Symposium on the Biology of Actinomycetes
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物細胞との共培養による植物内生放線菌の分離2016

    • Author(s)
      池田 翔一, 武 晃、塩見和朗, 大村智, 高橋洋子, 松本厚子
    • Organizer
      日本放線菌学会
    • Place of Presentation
      東京大学農学部弥生講堂、東京都文京区
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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