| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
During meiosis, most chromosomes have homologous partners and undergo homologous pairing mediated by the synaptonemal complex. In some organisms, the remaining unpaired chromosomes are heterochromatinized by unpaired silencing. Using the nematode C. elegans, we analyzed the differences and similarities of regulation of paired and unpaired chromosomes during meiosis.
We found that a cohesin containing COH-3/4 is involved not only in homolog pairing but also in unpaired silencing. In addition, CEC-5 possessing a chromodomain acts as a heterochromatin-specific factor in unpaired silencing. Heterochromatinization of unpaired chromosome is known to be abnormal in some RNAi mutants, such as the csr-1 mutant that corresponds to an Argonaute protein bearing an RNA cleavage activity. A defect in homologous pairing was also observed in the csr-1 mutant. Thus, mechanisms of RNAi, meiotic silencing and homologous pairing appear to overlap partially with each other in this organism.
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