Spatiotemporal fine tuning of mRNA stability and translation by RNA-binding proteins
| Project/Area Number |
15K06944
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
IRIE KENJI 筑波大学, 医学医療系, 教授 (90232628)
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| Co-Investigator(Renkei-kenkyūsha) |
SUDA YASUYUKI 筑波大学, 医学医療系, 助教 (10553844)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ＲＮＡ / 出芽酵母 / ポリＡ分解酵素 / 細胞壁 / ＲＮＡ結合タンパク質 / ポリA分解酵素 / 細胞壁合成 / 低分子量Gタンパク質 / ポリA鎖 / mRNA / RNA分解 / polyA鎖 |
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| Outline of Final Research Achievements |
Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. In the log-phase ccr4 mutant cells, LRG1 poly(A) tail was longer and LRG1 mRNA level was higher than those in the log-phase wild-type (WT) cells. Unexpectedly, Lrg1 protein level in the ccr4 mutant cells was comparable with that in WT. In the stationary-phase ccr4 mutant cells, LRG1 poly(A) tail length was still longer and LRG1 mRNA level was still higher than those in WT cells. In contrast to the log phase, Lrg1 protein level in the stationary-phase ccr4 mutant cells was maintained much higher than that in the stationary-phase WT cells. Loss of PBP1 reduced the LRG1 poly(A) tail length as well as LRG1 mRNA and protein levels in the stationary-phase ccr4 mutant cells. Our results suggest that Ccr4 regulates not only LRG1 mRNA level through poly(A) shortening but also the translation of LRG1 mRNA, and that Pbp1 is involved in the Ccr4-mediated regulation.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Evaluation of an Epitypified Ophiocordyceps formosana (Cordyceps s.l.) for Its Pharmacological Potential.2015
Author(s)
Wang YW, Hong TW, Tai YL, Wang YJ, Tsai SH, Lien PT, Chou TH, Lai JY, Chu R, Ding ST, Irie K, Li TK, Tzean SS, Shen TL.
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Journal Title
Evidence-Based Complementary and Alternative Medicine
Volume: 2015
Pages: 189891-189891
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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