| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
piRNAs bind PIWI proteins to control transposons to maintain germline genome integrity. piRNA intermediates are bound with PIWI anchored onto mitochondrial PAPI. However, whether Zucchini (Zuc) endonuclease, which play pivotal roles in Drosophila piRNA biogenesis, are required for this remains elusive.
We show that loss of Zuc in Bombyx aberrantly accumulated piRNA intermediates within the PAPI complex. PAPI exerted its RNA-binding activity only when being bound with PIWI and phosphorylated, suggesting a hierarchical process of the complex assembly. Both 5′ and 3′ ends of piRNA intermediates showed the hallmark of PIWI-Slicer. The 5′ end of Bombyx piRNA is formed by PIWI-Slicer, while the 3′ end is formed by Zuc endonuclease. The Bombyx piRNA biogenesis is simpler than that of Drosophila, because Bombyx has no phased piRNAs.
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