Chromatin reprogramming induces functional switching of DNA methylation enzyme
Project/Area Number |
15K06950
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | Toho University (2017) Tottori University (2015-2016) |
Principal Investigator |
TADA Masako 東邦大学, 理学部, 教授 (10524910)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | エピジェネティクス / DNAメチル化 / ES細胞 / クロマチン / 胚発生 / 細胞分化 / リプログラミング / Dnmt / Dnmt1 / マウスES細胞 / 5-ヒドロキシメチルシトシン / 5-ヒドロキシメチル化 |
Outline of Final Research Achievements |
Accurate control of DNA methylation (5mC) is essential for mammalian embryogenic development. ES cells lacking all three types of DNA methyltransferases, de novo-type Dnmt3a and Dnmt3b and maintenance-type Dnmt1 can proliferate, but die immediately after cell differentiation. When Dnmt1 was expressed in the TKO ESCs, the TKO+1 ESCs became possible to differentiate. The differentiated cell genomes became gradually methylated, and Tet enzyme-mediated oxidation products of 5mC were also detected. DNA methylation occurred even in the undifferentiated state when compounds capable of chromatin activation were added to the cell culture medium. These results indicate that Dnmt1 possesses de novo DNA methylation activity as an innate function that appears only when genomes become extensively reprogrammed.
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Report
(4 results)
Research Products
(26 results)
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[Presentation] Stepwise reprogramming of global histone and DNA marks during chicken embryonic development.2015
Author(s)
Tada, M.*, Hayashi, A., Asano, Y., Kubiura, M., Ishishita, S., Hori, H., Kimura, H., Fukagawa, T., Matsuda, Y.
Organizer
The 40th Naito Conference
Place of Presentation
Sapporo, Japan
Year and Date
2015-09-17
Related Report
Int'l Joint Research
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[Presentation] Coordinated regulation of cyclic re-methylation by Dnmt1 and Dnmt3a/3b after demethylation through 5-hydroxymethylation and differentiation in mouse embryonic stem cells.2015
Author(s)
Lefebvre, L., Kubiura, M., Bogutz, A., Kimura, H., Tajima, S., and Tada, M.*
Organizer
The 40th Naito Conference
Place of Presentation
Sapporo, Japan
Year and Date
2015-09-16
Related Report
Int'l Joint Research
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[Presentation] Stepwise epigenetic reprogramming during chicken embryonic development. Plenary lectures: New Technology in Chromosome Research.2015
Author(s)
Tada, M.*, Hayashi, A., Asano, Y., Kubiura, M., Ishishita, S., Hori, H., Kimura, H., Fukagawa, T., Matsuda, Y.
Organizer
The 5th Asian Chromosome Colloquium
Place of Presentation
Kasetsart University, Bangkok, Thailand
Year and Date
2015-04-29
Related Report
Int'l Joint Research
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