| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Centromere formation is epigenetically regulated so that one chromosome has one centromere, but the molecular mechanisms to limit the centromeres remain unclear. We attempted to understand the mechanisms by using fission yeast harboring fused two chromosomes. The analysis of chromatin revealed that histone H3 lysine 9 acetylation and histone H4 lysine 20 methylation that thought to induce the centromeric chromatin formation were suppressed by heterochromatin on the inactivated centromere. Furthermore, heterochromatin partially rescues defects caused by overexpression of the genes that mediate the loading of CENP-A, centromere specific histone H3 variant, into chromatin in the fission yeast harboring fused chromosome. These results suggested that the heterochromatin contribute to the restriction of excess centromeres formed on a chromosome.
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