| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Condensin I is a five-subunit protein complex that plays a central role in mitotic chromosome assembly in eukaryotes. To dissect its molecular mechanism of action, we had established a functional assay that combines reconstituted recombinant complexes and Xenopus egg extracts. In the current study, we investigated the role of CAP-H, the kleisin subunit of this complex, by introducing mutations in its conserved subdomains. We found that two sets of the subdomain of CAP-H, referred to as ‘motifs III and IV’, have functional and structural contributions to condensin I’s essential function in mitotic chromosome organization.
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