Photo-activated structural changes of bacterial sensory rhodopsin as revealed by photo-irradiation solid-state NMR

• Project/Area Number: 15K06963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama National University
• Principal Investigator: NAITO Akira 横浜国立大学, 大学院工学研究院, 名誉教授 (80172245)
• Co-Investigator(Renkei-kenkyūsha): KAWAMURA Izuru 横浜国立大学, 大学院工学研究院, 准教授 (20452047) ; SUDO Yuki 岡山大学, 医歯薬学部, 教授 (10452202)
• Research Collaborator: KAMO Naoki ; Wada Akimori
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 光受容膜タンパク質 / バクテリオロドプシン / センサリーロドプシン / レチナール発色団 / 光照射固体ＮＭＲ / ホボロドプシン / 光駆動プロトン輸送 / 光信号伝達 / 光駆動イオン輸送 / In situ光照射NMR / イオン輸送 / 信号伝達 / 光照射固体NMR

Outline of Final Research Achievements

It is essential to elucidate the photoreaction cycles to understand the function of photoreceptor membrane proteins. In this study, we have developed photo-irradiation solid-state NMR spectrometer to detect the photo-reaction pathways of photoreceptor membrane proteins.
The photoreaction pathways of Y185F-bR were examined using photo-irradiation solid-state NMR Under irradiation with 520 nm light, the CS state changed to a CS*-intermediate and AT state transformed to an N-intermediate and transformed to an O-intermediate.
The photoreaction pathways of ppR/pHtrII complex were examined using photo-irradiation solid-state NMR. The M-, O- and N’-intermediates were observed by illuminating with green light. Under blue light irradiation, the M-intermediates transformed to the O-intermediate which is in equilibrium with the N’-intermediate.

Research Products

• [Journal Article] Dynamic membrane interactions of antibacterial and antifungal biomolecules, and amyloid peptides, revealed by solid-state NMR spectroscopy (2018)
  • Author(s): Naito Akira、Matsumori Nobuaki、Ramamoorthy Ayyalusamy
  • Journal Title: Biochimica et Biophysica Acta (BBA) - General Subjects Volume: 1862 Issue: 2 Pages: 307-323
  • DOI: 10.1016/j.bbagen.2017.06.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quantitative Analysis of Solid-State Homonuclear Correlation Spectra of Antiparallel beta-Sheet Alanine Tetramers (2018)
  • Author(s): A. Naito, K. Okushita, K. Nishimura, G. S. Boutis, A. Aoki, and T. Asakura
  • Journal Title: J. Phys. Chem. B Volume: 122 Issue: 10 Pages: 2715-2724
  • DOI: 10.1021/acs.jpcb.7b11126
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The role of d - allo -isoleucine in the deposition of the anti- Leishmania peptide bombinin H4 as revealed by 31 P solid-state NMR, VCD spectroscopy, and MD simulation (2018)
  • Author(s): Mijiddorj Batsaikhan、Kaneda Shiho、Sato Hisako、Kitahashi Yuki、Javkhlantugs Namsrai、Naito Akira、Ueda Kazuyoshi、Kawamura Izuru
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Volume: 1866 Issue: 7 Pages: 789-798
  • DOI: 10.1016/j.bbapap.2018.01.005
  • NAID: 120006649291
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 生体幕内でのペプチドの挙動の分子動力学シミュレーション (2018)
  • Author(s): 上田 一義、内藤 晶、川村 出
  • Journal Title: 酵素工学ニュース Volume: 79 Pages: 27-30
  • Peer Reviewed
• [Journal Article] 固体高分解能NMRスペクトルに現れる異方性相互作用-Narrrow is beautifull- (2018)
  • Author(s): 内藤 晶
  • Journal Title: 分光研究 Volume: 67 Pages: 13-15
  • Peer Reviewed
• [Journal Article] Retinal configuration of ppR intermediates revealed by photo-irradiation solid-state NMR and DFT (2018)
  • Author(s): Makino Yoshiteru, Kawamura Izuru, Okitsu Takashi, Wada Akimori, Kamo Naoki, Sudo Yuki, Ueda Kazuyoshi, Naito Akira
  • Journal Title: Biophysical Journal Volume: in press
  • NAID: 120006698118
  • Peer Reviewed
• [Journal Article] Dynamic structure and orientation of melittin bound to acidic lipid bilayers, as revealed by solid-state NMR and molecular dynamic simulation (2017)
  • Author(s): K. Norisada, N. Javkhlantugs, D. Mishima, I. Kawamura, H. Saito, K. Ueda, A. Naito
  • Journal Title: J. Phys. Chem. B. Volume: 121 Issue: 8 Pages: 1802-1811
  • DOI: 10.1021/acs.jpcb.6b11207
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR (2017)
  • Author(s): Asakura Tetsuo、Miyazawa Kenta、Tasei Yugo、Kametani Shunsuke、Nakazawa Yasumoto、Aoki Akihiro、Naito Akira
  • Journal Title: Physical Chemistry Chemical Physics Volume: 19 Issue: 20 Pages: 13379-13386
  • DOI: 10.1039/c7cp01199g
  • Peer Reviewed
• [Journal Article] Refined Crystal Structure of Samia cynthia ricini Silk Fibroin Revealed by Solid-State NMR Investigations (2017)
  • Author(s): Asakura Tetsuo、Nishimura Akio、Kametani Shunsuke、Kawanishi Shuto、Aoki Akihiro、Suzuki Furitsu、Kaji Hironori、Naito Akira
  • Journal Title: Biomacromolecules Volume: 18 Issue: 6 Pages: 1965-1974
  • DOI: 10.1021/acs.biomac.7b00441
  • Peer Reviewed
• [Journal Article] Growth-incompetent monomers of human calcitonin lead to a noncanonical direct relationship between peptide concentration and aggregation lag time (2017)
  • Author(s): Kamgar-Parsi Kian、Hong Liu、Naito Akira、Brooks Charles L.、Ramamoorthy Ayyalusamy
  • Journal Title: Journal of Biological Chemistry Volume: 292 Issue: 36 Pages: 14963-14976
  • DOI: 10.1074/jbc.m117.791236
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Packing Arrangements and Intersheet Interaction of Alanine Oligopeptides As Revealed by Relaxation Parameters Obtained from High-Resolution 13C Solid-State NMR (2017)
  • Author(s): Naito Akira、Tasei Yugo、Nishimura Akio、Asakura Tetsuo
  • Journal Title: The Journal of Physical Chemistry B Volume: 121 Issue: 38 Pages: 8946-8955
  • DOI: 10.1021/acs.jpcb.7b07068
  • Peer Reviewed
• [Journal Article] Packing arrangement of 13C selectively labeled sequence model peptides of samia Cynthia ricini silk fibroin fiber studied by solid-state NMR (2017)
  • Author(s): T. Asakura, K. Miyazawa, Y. Tasei, S. Kametani, Y. Nakazawa, A. Aoki, A. Naito
  • Journal Title: Phys. Chem. Chem. Phys. Volume: in press
  • Peer Reviewed
• [Journal Article] Parallel b-sheet structure of alanine tetrapeptide in the solid state as studied by solid-state NMR spectroscopy (2016)
  • Author(s): T. Asakura, K. Horiguchi, A. Aoki, Y. Tasei, A. Naito
  • Journal Title: J. Phys. Chem B. Volume: 120 Issue: 34 Pages: 8912-8941
  • DOI: 10.1021/acs.jpcb.6b06292
  • Peer Reviewed
• [Journal Article] Structural biology of calcitonin: from aqueous therapeutic properties to amyloid aggregation (2016)
  • Author(s): K Kamgar-Parsi, J. Tolchard, B. Habenstein, A. Loquest, A. Naito, A. Ramamoorthy
  • Journal Title: Isr. J. Chem. Volume: in press Issue: 7-8 Pages: 634-650
  • DOI: 10.1002/ijch.201600096
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Polysilsesquioxanes with mixed self-assembled organic tethers: Alkyl chains and alkanoate-aminopropyl pairs (2016)
  • Author(s): H. Yoshitake, T. Kodate, T.Takagi, I. Kawamura, A. Naito
  • Journal Title: Reactive and Functional Polymers Volume: 99 Pages: 9-16
  • DOI: 10.1016/j.reactfunctpolym.2015.12.002
  • Peer Reviewed
• [Journal Article] Mechanism for microwave heating of 1-(4'-cyanophenyl)-4-propylcyclohexane characterized by in situ microwave irradiation NMR spectroscopy. (2015)
  • Author(s): Y. Tasei, T. Yamakami, I. Kawamura, T. Fujito, K. Ushida, M. Sato, A. Naito
  • Journal Title: J. Magn. Reson. Volume: 254 Pages: 27-34
  • DOI: 10.1016/j.jmr.2015.02.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The microwave heating mechanism of N-(4-methoxybenzyliden)-4-butylaniline in liquid crystalline and isotropic phase as determined using in situ microwave irradiation NMR spectroscopy. (2015)
  • Author(s): Y. Tasei, F. Tanigawa, I. Kawamura, T. Fujito, M. Sato, A. Naito
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 17 Issue: 14 Pages: 9082-9089
  • DOI: 10.1039/c5cp00476d
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of photo-intermediates in the photo-reaction pathways of a bacteriorhodopsin Y185F mutant using in situ photo-irradiation solid-state NMR spectroscopy (2015)
  • Author(s): Kyosuke Oshima, Aris Shigeta, Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Tuzi, Tetsuo Iwasa, Akira Naito
  • Journal Title: Photochem. Photobiol. Sci. Volume: 14 Issue: 9 Pages: 2015-2015
  • DOI: 10.1039/c5pp00154d
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recent solid-atate NMR studies of membrane-bound peptides and proteins (2015)
  • Author(s): Akira Naito, Izuru Kawamura, Namsrai Javkhlantugs,
  • Journal Title: Annu. Rep. NMR Spectrosc., Volume: 86 Pages: 333-411
  • DOI: 10.1016/bs.arnmr.2015.06.001
  • ISBN: 9780128021231
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structure and orientation of antibiotic peptide alamethicin in phospholipid bilayers as revealed by chemical shift oscillation analysis of solid state nuclear magnetic resonance and molecular dynamics simulationfion (2015)
  • Author(s): Takashi Nagao, Daisuke Mishima, Namsrai Javlhalantugs, Jun Wang, Daisuke Ishioka, Kiyonori Yokota, Kazushi, Norisada, Izuru Kawamua, Kazuyoshi, Ueda, Akira Naito
  • Journal Title: Biochim. Biophys. Acta Volume: 1848 Issue: 11 Pages: 2789-2798
  • DOI: 10.1016/j.bbamem.2015.07.019
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Dynamic membrane bound structure of melittin and alamethicin as revealed by solid-stae NMR and MD simulation (2018)
  • Author(s): A. Naito, T. Nagao, K. Norisada, N. Javkhlantugs, D. Mishima, I. Kawamura, K. Ueda
  • Organizer: Biophysical Society 62nd Annual Meeting, San Francisco, USA
  • Int'l Joint Research
• [Presentation] Microwave irradiation solid-state NMR spectroscopy to reveal microwave heating and energyflow mechanism of liquid crystals (2017)
  • Author(s): A. Naito
  • Organizer: The 7th Asia Pacific NMR Symposium, Bangalore (India)
  • Int'l Joint Research / Invited
• [Presentation] マイクロ波および光照射固体ＮＭＲの開発と液晶および光受容膜タンパク質への応用 (2017)
  • Author(s): 内藤 晶
  • Organizer: 第61回 固体ＮＭＲ・材料フォーラム、理化学研究所、横浜
  • Invited
• [Presentation] 13C固体ＮＭＲ（ＤＡＲＲ）を用いた13C選択標識エリ蚕絹モデルペプチドのパッキング様式の解析 (2017)
  • Author(s): 内藤 晶、青木昭宏、宮澤健太、田制侑悟、朝倉哲郎
  • Organizer: 第66回高分子学会年次大会、幕張メッセ
• [Presentation] Photo-intermediates in the photo-reaction pathways of bacteriorhodopsin as revealed by in-situ photo-irradiation solid-state NMR (2017)
  • Author(s): K. Oshima, Y. Otani, A. Shigeta, Y. Makino, I. Kawamura, T. Okitsu, A. Wada, S. Tuzi, T. Iwasa, A. Naito
  • Organizer: ISMAR2017, Quebrc, Canada
  • Int'l Joint Research
• [Presentation] Dynamic structure and orientation of Melittin and alamethicin as revealed by solid-state NMR and MD simulation (2017)
  • Author(s): A. Naito, T. Nagao, K. Norisada, N. Javkhlantugs, D. Mishima, D. Ishioka, K. Yokota, J. Wong, I. Kawamura, H. Saito, K. Ueda
  • Organizer: ISMAR2017, Quebrc, Canada
  • Int'l Joint Research
• [Presentation] Pressure and photo induced structural changes of photoreceptor proteins as revealed by solid-state NMR (2017)
  • Author(s): A. Naito
  • Organizer: IMBP2017, Kyoto (Japan)
  • Int'l Joint Research / Invited
• [Presentation] Membrane binding structure of Bombinin H2 and H4 peptides in leishmanial mimetic membrane as studied by solid-state NMR and MD simulation (2017)
  • Author(s): M. Batsaikhan, S. Kaneda, N. Javkhalantugs, K. Ueda, H. Sato, A. Naito, I. Kawamura
  • Organizer: 第55回生物物理学会年会、熊本
• [Presentation] In situ光照射固体ＮＭＲによるバクテリオロドプシンとその変異体に生成する光反応中間体の定常捕捉 (2017)
  • Author(s): 内藤 晶、大島恭介、大谷優人、重田安里寿、槇野義輝、川村 出、沖津貴志、和田昭盛、辻 暁、岩佐達郎
  • Organizer: 第55回生物物理学会年会、熊本
• [Presentation] 13C NMR 化学シフト計算によるセンサリーロドプシンの光中間体におけるレチナール構造の解析 (2017)
  • Author(s): Y. Makino, I. Kawamura, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, A. Naito, K. Ueda
  • Organizer: 第55回生物物理学会年会、熊本
• [Presentation] Microwave heating mechanism of liquid crystals as revealed by microwave irradiation NMR (2017)
  • Author(s): A. Naito
  • Organizer: Magnetism and Magnetic Materials, London (UK)
  • Int'l Joint Research / Invited
• [Presentation] Photoirradiation and microwave irradiation NMR spectroscopy. Application to photoreceptor retinal proteins and liquid crystals (2017)
  • Author(s): A. Naito
  • Organizer: Taiwan-Japan Biomedical Symposium on Magnetic Resonance, Tainan (Taiwan)
  • Int'l Joint Research / Invited
• [Presentation] センサリーロドプシンのレチナール構造変化に対する13C NMR化学シフト値計算 (2017)
  • Author(s): 槇野義輝、川村 出、沖津貴志、和田昭盛、須藤雄気、加茂直樹、内藤 晶、上田一義
  • Organizer: 第５６回ＮＭＲ討論会(2017)、首都大学東京
• [Presentation] 固体ＮＭＲによるPhe-Pheモチーフをもつペプチドの自己組織化集合体の分子構造の解析 (2017)
  • Author(s): 尾崎夢実、Batsaikhan, Mijiddori, 佐藤 久子、内藤 晶、川村 出
  • Organizer: 第５６回ＮＭＲ討論会(2017)、首都大学東京
• [Presentation] マイクロ波照射ＮＭＲ分光法による液晶分子のマイクロ波加熱分子機構の解明 (2017)
  • Author(s): 内藤 晶、田制侑悟、槇野義輝、藤戸輝明、川村 出
  • Organizer: 第５６回ＮＭＲ討論会(2017)、首都大学東京
• [Presentation] Secondary structural change of glucagon during fibril formation process with DMPC lipid bilayers as revealed by 13C solid-state NMR (2016)
  • Author(s): K. Haya, I. Kawamura, A. Naito
  • Organizer: 第54回生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Structure of Tyr185 in dark-adapted bacteriorhodopsin as studied by solid-state NMR (2016)
  • Author(s): Y. Otani, A. Shigeta, Y. Kebukawa, K. Kobayashi, T. Okitsu, A. Wada, S. Tuzi, A. Naito, I. Kawamura
  • Organizer: 第54回生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Specific interaction of cationic antimicrobial peptide bombinin H2 and H4 with Leishmania protozoa mimetic membrane (2016)
  • Author(s): S. Kaneda, A. Naito, I. Kawamura
  • Organizer: 第54回生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Structure and orientation of antimicrobial peptides alamethicin and melittin in membrane revealed by solid state NMR and MD simulation (2016)
  • Author(s): A. Naito, T. Nagao, D. Mishima, N. Javkhlantugs, J. Wang, D. Ishioka, K. Yokota, K. Norisada, I. Kawamura, K. Ueda
  • Organizer: 第54回生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] Characterization of photo intermediates in sensory rhodopsin II as revealed by in-situ photoirradiation solid-state NMR (2016)
  • Author(s): Y. Makino, I. Kawamura, T. Okitsu, A. Wada, Y. Sudo, N. Kamo, A. Naito, K. Ueda
  • Organizer: 第54回生物物理学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-11-25
• [Presentation] High resolution solid-state NMR spectroscopy. Development and application to structural biochemistry (2016)
  • Author(s): A. Naito
  • Organizer: 第55回ＮＭＲ討論会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-16
  • Invited
• [Presentation] １３C固体NMRを用いたDMPC脂質二重膜存在下におけるペプチドホルモングルカゴンの線維構造の解析 (2016)
  • Author(s): 羽矢和未、川村 出、内藤 晶
  • Organizer: 第55回ＮＭＲ討論会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-16
• [Presentation] 光照射固体NMRによるセンサリーロドプシンIIの光反応過程における光中間体構造の解明 (2016)
  • Author(s): 槙野義輝、川村 出、沖津貴志、和田昭盛、須藤勇雄気、加茂直樹、内藤 晶、上田一義
  • Organizer: 第55回ＮＭＲ討論会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-16
• [Presentation] In-situ光照射固体NMRによるバクテリオロドプシンD96N変異体の光反応過程でのレチナールおよびタンパクシ質構造変化の解析 (2016)
  • Author(s): 大谷優人、重田 安里寿、 槙野義輝、宮佐亮太、川村 出、沖津貴志、和田昭盛、辻 暁、内藤 晶
  • Organizer: 第55回ＮＭＲ討論会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-16
• [Presentation] ３１P固体NMRによる抗菌ペプチドボンビニンH2およびH4の細菌模倣膜に対する作用の解析 (2016)
  • Author(s): 金田志穂、北橋由紀、内藤 晶、川村 出
  • Organizer: 第55回ＮＭＲ討論会
  • Place of Presentation: 広島
  • Year and Date: 2016-11-16
• [Presentation] Photoreaction pathways of bacteriorhodopsin and its mutants as revealed by in-situ photo-irradiation solid-state NMR (2016)
  • Author(s): A. Naito,, Y. Makino, K. Oshima, Y. Otani, A. Shigeta, I. Kawamura, T. Okitsu, A. Wada, S. Tuzi,, T. Iwasa
  • Organizer: ICRP2016
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2016-10-02
  • Int'l Joint Research
• [Presentation] Structure and orientation of antibiotic peptide alamethicin in membrane environment as revealed by chemical shift oscillation analysis of solid state NMR and MD simulation (2016)
  • Author(s): T. Nagao, D. Mishima, N. Javkhalantugs, J. Wang, D. Ishioka, K. Yokota, K. Norisada, I. Kawamura, K. Ueda, A. Naito
  • Organizer: ICBRBS2016
  • Place of Presentation: Kyoto
  • Year and Date: 2016-08-21
  • Int'l Joint Research / Invited
• [Presentation] Analysis of interaction of antimicrobial peptides bombinin H2 and H4 with the mimetic bacterial membrane by 31P solid-state NMR (2016)
  • Author(s): S. Kaneda, Y. Kitahashi, I. Kawamura, A. Naito
  • Organizer: ICBRBS2016
  • Place of Presentation: Kyoto
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Structural change of Tyr185 in the vicinity of the retinal chromophore in bacteriorhodopsin by 13C solid-state NMR (2016)
  • Author(s): Y. Otani, A. Shigeta, Y. Kebukawa, K. Kopbayashi, T. Okitsu, A. Wada, S. Tuzi, A. Naito, I. Kawamura
  • Organizer: ICBRBS2016
  • Place of Presentation: Kyoto
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Structural changes of retinal in sensory rhodopsin II as revealed by in situ photoirradiation solid-state NMR (2016)
  • Author(s): Y. Makino, I. Kawamura, T. Okitsu, A. Wada, S. Sudo, N. Kamo, A. Naito, I. Kawamura, K. Ueda
  • Organizer: ICBRBS2016
  • Place of Presentation: Kyoto
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] 固体NMRとMDシミュレーションによるリン脂質二分子膜に結合した抗生物質ペプチドアラメチシンの構造と配向の解明 (2016)
  • Author(s): 永尾隆、三島大輔、ジャブカランタグス ナムズライ、王 クン、石岡大輔、横田恭宣、則定和志、川村 出、上田一義、内藤 晶
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-06-07
• [Presentation] Photoreaction pathways of photoreceptor membrane proteins revealed by in-situ photo-irradiation solid-state NMR (2015)
  • Author(s): Akira Naito
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Solid-state NMR studies for chromophore interaction changes in pharaonis phoborhodopsin (2015)
  • Author(s): Izuru Kawamura, Ryota Nishikawa,Takashi Okitsu, Akimori Wada, Naoki Kamo
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Interaction of bombinin H4 containing a D-amino acid residue with liquid membranes as studied by solid-state NMR and MD simulations (2015)
  • Author(s): Yuki Kitahashi, Namsrai Javkhlantugs, Shiho Kaneda, Nyamsambuu,Altannavch, Kazuyoshi Ueda, Akira Naito, Izuru Kawamura
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu、Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Hydrogen bond alteration of Tyr174 and Tyr199 of phoborhodopsin in the lipid environment as studied by solid-state 13C NMR (2015)
  • Author(s): Ryota Nishikawa, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Yuki Sudo, Naoki Kamo, Akira Naito
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Structural changes in the photo-reaction pathways of retinal of bacteriorhodopsin studied by in-situ photo-irradiation solid-state NMR (2015)
  • Author(s): Aris Shigeta, Kyosuke Oshima, Ryota Miyasa, Miyako Horigome, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Tuzi, Akira Naito
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Structural diversity of amyloid fibril and its intermediate in human calcitonin as studied by solid-state NMR and MD simulation (2015)
  • Author(s): Shuhei Toyoda, Lkhansuren Ganchimegs, Namsrai Javkhlantugs, Hikari-Itoh-Watanabe, Izuru Kawamura, Kazuyoshi Ueda, Akira Naito
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Photo reaction pathways of sensory rhodopsin as revealed by in-situ photo-irradiation solid-state NMR (2015)
  • Author(s): Yoshiteru Makino, Hiroki Yomoda, Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Yuki Sudo, Akira Naito
  • Organizer: 2015 International Chemical Congress of Pacific Basin Society (Pacifichem 2015),
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] In-situ光照射固体NMRによるD96N-bR変異体の光反応サイクルにおけるM-中間体の解析 (2015)
  • Author(s): 大谷優人、重田安里寿、槙野義輝、宮佐亮太、川村 出、沖津貴志、和田昭盛、辻暁、内藤 晶
  • Organizer: 第５４回NMR討論会
  • Place of Presentation: 千葉工業大学
  • Year and Date: 2015-11-06
• [Presentation] 13-cis, 15-syn型バクテリオロドプシンの光中間体のin-situ光照射固体NMRによる検出 (2015)
  • Author(s): 重田安里寿、大島恭介、川村 出、沖津貴志、和田昭盛、辻暁、内藤晶
  • Organizer: 第５４回NMR討論会
  • Place of Presentation: 千葉工業大学
  • Year and Date: 2015-11-06
• [Presentation] In situ光照射固体NMRによる光受容膜タンパク質ｐｐR/ｐHtrIIの光活性中間体の解析 (2015)
  • Author(s): 槙野義輝、友永雄也、日高哲郎、川村 出、沖津貴志、和田昭盛、須藤雄気、加茂直樹、内藤 晶
  • Organizer: 第５４回NMR討論会
  • Place of Presentation: 千葉工業大学
  • Year and Date: 2015-11-06
• [Presentation] In situ光照射固体NMRによるバクテリオロドプシンY185F変異体の光反応経路の解明 (2015)
  • Author(s): 大島恭介、重田安里寿、槙野義輝、川村 出、沖津貴志、和田昭盛、辻暁、岩佐達郎、内藤 晶
  • Organizer: 第５３回日本生物物理学会年会
  • Place of Presentation: 金沢大学
  • Year and Date: 2015-09-13
• [Presentation] The microwave heating mechanism of MBBA as studied by microwave irradiation NMR spectroscopy (2015)
  • Author(s): Akira Naito, Yugo Tasei, Teruaki Fujito, Izuru Kawamura
  • Organizer: The 19th International Society of Magnetic Resonance Conference
  • Place of Presentation: Shanghai, China
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] 13C solid-state NMR study of hydrogen-bonding alteration of Tyr174 and Tyr199 of phoborhodopsin in the lipid environments (2015)
  • Author(s): Izuru Kawamura, Ryota Nishikawa, Takashi Okitsu, Akimori Wada, Yuki Sudo, Naoki Kamo, Akira Naito
  • Organizer: The 19th International Society of Magnetic Resonance Conference
  • Place of Presentation: Shanhai, China
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] Color-discriminating retinal configuration changes of sensory rhodopsin I as revealed by in situ photo-irradiation solid-state NMR (2015)
  • Author(s): Yoshiteru Makino, Hiroki Yomoda,, Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Takashi Okitsu, Akimori Wada,Yuki Sudo, Akira Naito
  • Organizer: The 19th International Society of Magnetic Resonance Conference
  • Place of Presentation: Shanghai, China
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] Structural changes in the photo-excited process of bacteriorhodopsin studied by photo-irradiation solid-state NMR (2015)
  • Author(s): Arisu Shigeta, Kyosuke Oshima, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Tuzi, Akira Naito
  • Organizer: The 19th International Society of Magnetic Resonance Conference
  • Place of Presentation: Shanghai, Chaina
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] Determination of photo-intermediates and photo-reaction pathways of photoreceptor membrane proteins by in-situ photo-irradiation solid-state NMR (2015)
  • Author(s): Akira Naito, Yoshiteru Makino, Hiroki Yomoda, Yuya Tomonaga, Tetsurou Hidaka, Izuru Kawamura, Yuki Sudo, Akimori Wada, Takashi Okitsu, Naoki Kamo
  • Organizer: The 6th Asia-Pacific NMR Symposium
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2015-08-13
  • Int'l Joint Research / Invited
• [Presentation] 固体NMRによる抗菌ペプチドの生体膜結合構造と膜相互作用解析 (2015)
  • Author(s): 内藤 晶
  • Organizer: 第15回 日本蛋白質科学会年会
  • Place of Presentation: あわぎんホール、徳島
  • Year and Date: 2015-06-24
  • Invited
• [Book] Photoirradiation and microwave irradiation NMR. Experimental Approaches of NMR Spectroscopy. Methodology and Applicatioin to Life Science and Material Science (2018)
  • Author(s): A. Naito, Y. Makino, Y. Tasei, I. Kawamura
  • Total Pages: 36
  • Publisher: Springer
• [Book] Structure determination of membrane peptides and proteins by solid-state NMR. Experimental Approaches of NMR Spectroscopy. Methodology and Applicatioin to Life Science and Material Science (2018)
  • Author(s): I. Kawamura, K. Norisada, A. Naito
  • Total Pages: 44
  • Publisher: Springer
• [Book] 生命科学のための物理化学15講 (2018)
  • Author(s): 功刀 滋、内藤 晶
  • Total Pages: 256
  • Publisher: 講談社
  • ISBN: 9784061538986
• [Book] In-situ photo irradiation solid-state NMR spectroscopy applied to retinal-binding membrane proteins. Modern Magnetic Resonance, (2017)
  • Author(s): A. Naito, Y. Makino, I. Kawamura
  • Total Pages: 22
  • Publisher: Springer International Publishing
• [Book] In-situ photo irradiation solid-state NMR spectroscopy applied to retinal-binding membrane protein, Modern Magn. Reson. (2017)
  • Author(s): A. Naito, Y. Makino, I. Kawamura
  • Publisher: Springer
• [Book] Photoirradiation and microwave irradiation NMR spectroscopy, Experimental approaches of NMR spectroscopy. ;Methodology and Application of Life Science and Material Science (2017)
  • Author(s): A. Naito, Y. Makino, Y. Tasei, I. Kawamura
  • Publisher: Springer
• [Book] 固体高分解能NMR(Narrow is beautiful)の構造生命化学研究への展開 NMR (Bulletin of the nuclear magnetic society of japan) (2016)
  • Author(s): 内藤 晶
  • Total Pages: 3
  • Publisher: 日本核磁気共鳴学会
• [Book] 光と生命の事典 第５章 光による生命現象の解明、第１７６節 ＮＭＲ分光法 (2015)
  • Author(s): 内藤 晶
  • Total Pages: 4
  • Publisher: 朝倉書店