| Project/Area Number |
15K06963
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama National University |
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Principal Investigator |
NAITO Akira 横浜国立大学, 大学院工学研究院, 名誉教授 (80172245)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMURA Izuru 横浜国立大学, 大学院工学研究院, 准教授 (20452047)
SUDO Yuki 岡山大学, 医歯薬学部, 教授 (10452202)
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| Research Collaborator |
KAMO Naoki
Wada Akimori
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光受容膜タンパク質 / バクテリオロドプシン / センサリーロドプシン / レチナール発色団 / 光照射固体ＮＭＲ / ホボロドプシン / 光駆動プロトン輸送 / 光信号伝達 / 光駆動イオン輸送 / In situ光照射NMR / イオン輸送 / 信号伝達 / 光照射固体NMR |
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| Outline of Final Research Achievements |
It is essential to elucidate the photoreaction cycles to understand the function of photoreceptor membrane proteins. In this study, we have developed photo-irradiation solid-state NMR spectrometer to detect the photo-reaction pathways of photoreceptor membrane proteins.
The photoreaction pathways of Y185F-bR were examined using photo-irradiation solid-state NMR Under irradiation with 520 nm light, the CS state changed to a CS*-intermediate and AT state transformed to an N-intermediate and transformed to an O-intermediate.
The photoreaction pathways of ppR/pHtrII complex were examined using photo-irradiation solid-state NMR. The M-, O- and N’-intermediates were observed by illuminating with green light. Under blue light irradiation, the M-intermediates transformed to the O-intermediate which is in equilibrium with the N’-intermediate.
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