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The structure analysis of PBCV-1 by the combination of X-ray crystallography and coherent X-ray diffraction imaging

Research Project

Project/Area Number 15K06971
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionOsaka University

Principal Investigator

Higashiura Akifumi  大阪大学, たんぱく質研究所, 助教 (90598129)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsX線結晶解析 / コヒーレントX線イメージング / 構造生物学
Outline of Final Research Achievements

Studies aimed at the structure analysis of a giant virus, PBCV-1 by the combination of X-ray crystallography and coherent X-ray diffraction imaging was carried out. The single crystals of PBCV1 have not been obtained yet, but the technical improvements were proceeded using the other virus crystals as a model. The high-resolution diffraction patterns were successfully observed from viral fragile crystals.
In the experiment of coherent X-ray diffraction imaging, so many diffraction patterns from PBCV-1 were collected and processed for 2D imaging. The data selection of diffraction data from huge data set is carrying out aimed to the 3D reconstruction of PBCV-1.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 4 results)

  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] Uppsala university(Sweden)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Hierarchical structure assembly model of rice dwarf virus particle formation.2018

    • Author(s)
      Nakagawa A., Miyazaki N., Higashiura A.
    • Journal Title

      Biophys Rev.

      Volume: 10(2) Issue: 2 Pages: 659-665

    • DOI

      10.1007/s12551-017-0375-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source2016

    • Author(s)
      Munke A.,…,Daurer B.J.,…,Hajdu J.,Hantke M.F.,Higashiura A.,…,Kumar H.,…,Maia F.R.N.C.,…,Nakagawa A.,…,Okamoto K., …,van der Schot G.,…,Seibert M.M.,…,Svenda M.,et al
    • Journal Title

      Scientific Data

      Volume: 3 Issue: 1 Pages: 160064-160064

    • DOI

      10.1038/sdata.2016.64

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Structure assembly mechanism of Rice Dwarf Virus2017

    • Author(s)
      Higashiura, A.; Nakamichi, Y.; Miyazaki, N.; Tsutsumi, K.; Iwasaki, K.; Murata, K.; Omura, T. & Nakagawa, A.
    • Organizer
      IUCr
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SPring-8 BL44XU, A Beamline Designed for Structure Analysis of Large Biological Macromolecular Assemblies2016

    • Author(s)
      Yamashita E., Higashiura A., Yoshimura M., Hasegawa K., Furukawa Y., Kumasaka T., Ueno G., Yamamoto M., Tsukihara T., Nakagawa A.
    • Organizer
      5th International symposium on diffraction structural biology
    • Place of Presentation
      Knoxville, US
    • Year and Date
      2016-08-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Approaches for Coherent X-ray Diffraction Imaging of Paramecium bursaria Chlorella virus-12015

    • Author(s)
      Akifumi Higashiura, Gijs van der Schot, Max Hantke, Jing Liu, Takashi Yamada, Janos Hajdu, Atsushi Nakagawa
    • Organizer
      The 13th Conference of the Asian Crystallographic Association
    • Place of Presentation
      Kolkata, India
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 高圧凍結法を用いたイネ萎縮ウイルスの高分解能X線結晶構造解析への取り組み2015

    • Author(s)
      東浦 彰史, 中道 優介, 太田 和敬, 宮崎 直幸, 一木 珠樹, 大村 敏博, 中川 敦史
    • Organizer
      平成27年度 日本結晶学会 年会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] SPring-8 BL44XU, Beamline Designed for Structure Analysis of Large Biological Macromolecular Assemblies2015

    • Author(s)
      Akifumi Higashiura, Eiki Yamashita, Masato Yoshimura, Kazuya Hasegawa, Yukito Furukawa, Takashi Kumasaka, Go Ueno, Masaki Yamamoto, Tomitake Tsukihara, Atsushi Nakagawa
    • Organizer
      12th International Conference on Synchrotron Radiation Instrumentation
    • Place of Presentation
      New York, USA
    • Year and Date
      2015-07-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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