Elucidation of the structural basis of transporter proteins involved in metal tolerance

• Project/Area Number: 15K06972
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: TANAKA Yoshiki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10632333)
• Co-Investigator(Renkei-kenkyūsha): TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
• Research Collaborator: IWAKI Shigehiro
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: タンパク質構造 / 膜タンパク質 / 構造解析 / 輸送体膜タンパク質 / 輸送体 / タンパク質 / イオン輸送 / X線結晶構造解析 / 構造生物学

Outline of Final Research Achievements

We conducted a crystal structure analysis on the multidrug efflux transporter MATE, which is a transporter related to plant metal tolerance, and reported its structure.
In the structure revealed, it became clear that some acidic amino acid side chains are concentrated in the inner pocket part. It was found that the aspect of the substrate binding region is different between the prokaryotic MATE which discharges hydrophobic substrates and the plant MATE which is related to emit cation such as aluminum.

Research Products

• [Journal Article] Remote Coupled Drastic β-Barrel to β-Sheet Transition of the Protein Translocation Motor. (2018)
  • Author(s): Furukawa A, Nakayama S, Yoshikaie K, Tanaka Y, Tsukazaki T.
  • Journal Title: Structure Volume: 26 Issue: 3 Pages: 485-489
  • DOI: 10.1016/j.str.2018.01.002
  • Peer Reviewed / Open Access
• [Journal Article] Crystal Structure of a Plant Multidrug and Toxic Compound Extrusion Family Protein (2017)
  • Author(s): Tanaka Yoshiki、Iwaki Shigehiro、Tsukazaki Tomoya
  • Journal Title: Structure Volume: 25 Issue: 9 Pages: 1455-1460
  • DOI: 10.1016/j.str.2017.07.009
  • Peer Reviewed / Open Access
• [Journal Article] Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF (2017)
  • Author(s): Arata Furukawa, Kunihito Yoshikaie, Takaharu Mori, Hiroyuki Mori, Yusuke V. Morimoto, Yasunori Sugano, Shigehiro Iwaki, Tohru Minamino, Yuji Sugita, Yoshiki Tanaka, Tomoya Tsukazaki
  • Journal Title: Cell Reports Volume: 19 Issue: 5 Pages: 1-7
  • DOI: 10.1016/j.celrep.2017.04.030
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] SecY-SecA fusion protein retains the ability to mediate protein transport (2017)
  • Author(s): Sugano Yasunori、Furukawa Arata、Nureki Osamu、Tanaka Yoshiki、Tsukazaki Tomoya
  • Journal Title: PLOS ONE Volume: 12 Issue: 8 Pages: e0183434-e0183434
  • DOI: 10.1371/journal.pone.0183434
  • Peer Reviewed / Open Access
• [Journal Article] BepA mediates productive transfer of substrate proteins to the β-barrel assembly machinery (BAM) complex. (2017)
  • Author(s): Daimon, Y., Masui, C., Tanaka, Y., Shiota, T., Suzuki, T., Miyazaki, R., Sakurada, H., Lithgow, T., Dohmae, N., Mori, H., Tsukazaki, T., Narita, S. and Akiyama, Y.
  • Journal Title: Mol. Microbiol. Volume: 106 Issue: 5 Pages: 760-776
  • DOI: 10.1111/mmi.13844
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crystal structures of SecYEG in lipidic cubic phase elucidate a precise resting and a peptide-bound states (2015)
  • Author(s): Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
  • Journal Title: Cell Reports Volume: 13 Pages: 15611568-15611568
  • NAID: 120005716877
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] MATEトランスポーターの結晶構造解析 (2018)
  • Author(s): 岩木 薫大, 田中 良樹, 塚崎 智也
  • Organizer: 第59回日本植物生理学会
• [Presentation] 膜輸送装置の構造と機能 (2017)
  • Author(s): 田中良樹
  • Organizer: 日本細菌学会
• [Presentation] Complete and peptide-bound structures of the Sec translocon (2015)
  • Author(s): Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
  • Organizer: 新学術領域研究「動的秩序と機能」第4回公開国際シンポジウム
  • Place of Presentation: 九州大学西新プラザ（福岡県福岡市）
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] 新生膜タンパク質の膜組込み過程の構造生物科学 (2015)
  • Author(s): 田中 良樹
  • Organizer: 新学術領域研究「新生鎖の生物学」 平成27年度第2回班会議
  • Place of Presentation: ほほえみの宿（山形県天童市）
  • Year and Date: 2015-11-14
• [Presentation] Structures of Membrane Protein Insertase YidC (2015)
  • Author(s): Tomoya Tsukazaki, Kaoru Kumazaki, Shinobu Chiba, Mizuki Takemoto, Arata Furukawa, Toshiki Kishimoto, Yasunori Sugano, Takaharu Mori, Yoshiki Tanaka, Yuji Sugita, Koreaki Ito, Ryuichiro Ishitani and Osamu Nureki
  • Organizer: Structural Biological Dynamics: From Molecules to Life with 60 trillion Cells
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-11-05
  • Int'l Joint Research
• [Presentation] Cytoplasmic conformational transition of Sec translocon (2015)
  • Author(s): Yoshiki Tanaka, Yasunori Sugano, Mizuki Takemoto, Takaharu Mori, Takamitsu Haruyama, Arata Furukawa, Tsukasa Kusakizako, Kaoru Kumazaki, Ayako Kashima, Ryuichiro Ishitani, Hiroki Konno, Yuji Sugita, Osamu Nureki and Tomoya Tsukazaki
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] 新生膜タンパク質の膜組込み過程の構造生物科学 (2015)
  • Author(s): 田中 良樹, 塚﨑 智也*, 古川新, 菅野泰功
  • Organizer: 新学術領域研究「新生鎖の生物学」 平成２７年度第一回班会議
  • Place of Presentation: 東京工業大学（東京都港区）
  • Year and Date: 2015-06-13